Abstract: A single-domain antibody or an antigen-binding fragment thereof for neutralizing canine parvovirus (CPV) including a heavy chain encompassing a variable region. The variable region includes three complementarity-determining regions (CDRs): CDR1, CDR2, and CDR3. The three complementarity-determining regions CDR1, CDR2, and CDR3 have a combination of different amino acid sequences. The antibodies effectively prevent CPV from infecting F81 cells, exhibiting high binding affinity and specificity, and significant neutralizing activity against CPV. Consequently, the antibody sequences and expression vectors of the disclosure are valuable tools for various applications in CPV epitope identification, prevention, treatment, and diagnosis.

Abstract: A T-cell epitope polypeptide of foot and mouth disease virus (FMDV) having an amino acid sequence represented by one of SEQ ID Nos: 1-11: SEQ ID NO: 1: KGKPFNSKVIIATTNLYS; SEQ ID NO: 2: PLQNVYQLVQEVIDRVEL; SEQ ID NO: 3: VVMDDLGQNPDGKDFKYF; SEQ ID NO: 4: YNQQTVVVMDDLGQNPDG; SEQ ID NO: 5: GRTDSVWYCPPDPDHFDG; SEQ ID NO: 6: RGKSGQGKSFLANVLAQA; SEQ ID NO: 7: PDFNRLVSAFEELATGVK; SEQ ID NO: 8: AIRTGLDEAKPWYKLIKL; SEQ ID NO: 9: MSTKHGPDFNRLVSAFEE; SEQ ID NO: 10: MLDGRTMTDSDYRVF; and SEQ ID NO: 11: VLDEVIFSKHKGDTK.

Abstract: A T-cell epitope polypeptide of Senecavirus A (SVA) having an amino acid sequence represented by one of SEQ ID NOs: 1-7: SEQ ID NO: 1: DEALGRVLTPAAVDEALVDL; SEQ?ID?NO:?2: AILAKLGLALAAVTPGLIIL; SEQ?ID?NO:?3: KASPVLQYQL; SEQ?ID?NO:?4: EMKKLGPVAL; SEQ?ID?NO:?5: AHDAFMAGSG; SEQ?ID?NO:?6: PPLGDDQIEYLQVLKSLALT; and SEQ?ID?NO:?7: LASTLIAQAVSKRLYGSQSV.

Abstract: A single-domain antibody or an antigen-binding fragment thereof to Senecavirus A, the single-domain antibody or the antigen-binding fragment thereof being selected from 52 amino acid sequence groups, and each of the 52 amino acid sequence groups including a first variable domain of heavy chain VH-CDR-1, a second variable domain of heavy chain VH-CDR-2, and a third variable domain of heavy chain VH-CDR-3. The first variable domain of heavy chain VH-CDR-1 has an amino acid sequence represented by one of SEQ ID NO: 1-SEQ ID NO: 52. The second variable domain of heavy chain VH-CDR-2 has an amino acid sequence represented by one of SEQ ID NO: 53-SEQ ID NO: 104. The third variable domain of heavy chain VH-CDR-3 has an amino acid sequence represented by one of SEQ ID NO: 105-SEQ ID NO: 156.

Abstract: A test strip for detecting a serotype O foot-and-mouth disease, the test strip including: a bottom board; a detection layer being disposed on the bottom board and including a detection line and a control line; an absorbent layer being disposed at one end of the detection layer close to the control line; a gold colloidal conjugate pad being disposed at the other side of the detection layer close to the detection line; and a sample pad is disposed on a top of the gold colloidal conjugate pad. The gold colloidal conjugate pad is coated with colloidal gold particles that are conjugated with a Staphylococcus protein A (SPA) marker. The detection line is coated or impregnated with serotype O FMDV-like particles, and the control line is coated or impregnated with rabbit IgG.

Abstract: A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.

Abstract: Foot-and-mouth disease virus like particles, the particles including a structural protein VP0, a structural protein VP1 including a mineralization peptide, a structural protein VP3, and a calcium phosphate coat. The structural protein VP1 including a mineralization peptide is encoded by a gene sequence represented by SEQ ID NO. 7, SEQ ID NO. 8 or SEQ ID NO. 9. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO. 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO. 3. The calcium phosphate coat covers the structural protein VP0, the structural protein VP1 including a mineralization peptide, and the structural protein VP3.

Abstract: A virus-like particle of Senecavirus A, the particle including a structural protein VP0, a structural protein VP1 and a structural protein VP3. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO: 1. The structural protein VP1 is encoded by a gene sequence represented by SEQ ID NO: 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO: 3.

Abstract: A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.

Abstract: A virus-like particle of Senecavirus A, the particle including a structural protein VP0, a structural protein VP1 and a structural protein VP3. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO: 1. The structural protein VP1 is encoded by a gene sequence represented by SEQ ID NO: 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO: 3.

Abstract: Foot-and-mouth disease virus like particles, the particles including a structural protein VP0, a structural protein VP1 including a mineralization peptide, a structural protein VP3, and a calcium phosphate coat. The structural protein VP1 including a mineralization peptide is encoded by a gene sequence represented by SEQ ID NO. 7, SEQ ID NO. 8 or SEQ ID NO. 9. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO. 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO. 3. The calcium phosphate coat covers the structural protein VP0, the structural protein VP1 including a mineralization peptide, and the structural protein VP3.