Patents by Inventor Huimin Kong

Huimin Kong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • ENDONUCLEASE-ENHANCED HELICASE-DEPENDENT AMPLIFICATION
    Publication number: 20160168630
    Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
    Type: Application
    Filed: February 25, 2016
    Publication date: June 16, 2016
    Inventors: Huimin Kong, Yanhong Tong
  • Endonuclease-enhanced helicase-dependent amplification
    Patent number: 9303288
    Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: April 5, 2016
    Assignee: BioHelix Corporation
    Inventors: Huimin Kong, Yanhong Tong
  • Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
    Patent number: 9121054
    Abstract: Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of test and control nucleic acids. Test nucleic acids can be immobilized at multiple locations, such that amplification of either a test nucleic acid or a control nucleic acid provides a captured nucleic acid in a control capture zone.
    Type: Grant
    Filed: December 7, 2010
    Date of Patent: September 1, 2015
    Assignee: BioHelix Corporation
    Inventors: Huimin Kong, Yanhong Tong, Wen Tang
  • ENDONUCLEASE-ENHANCED HELICASE-DEPENDENT AMPLIFICATION
    Publication number: 20140186847
    Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
    Type: Application
    Filed: March 4, 2014
    Publication date: July 3, 2014
    Applicant: BioHelix Corporation
    Inventors: Huimin Kong, Yanhong Tong
  • Endonuclease-enhanced helicase-dependent amplification
    Patent number: 8685648
    Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 1, 2014
    Assignee: BioHelix Corporation
    Inventors: Huimin Kong, Yanhong Tong
  • Endonuclease-Enhanced Helicase-Dependent Amplification
    Publication number: 20120040359
    Abstract: The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
    Type: Application
    Filed: February 3, 2010
    Publication date: February 16, 2012
    Inventors: Huimin Kong, Yanhong Tong
  • DETECTION OF NUCLEIC ACID AMPLIFICATION PRODUCTS IN THE PRESENCE OF AN INTERNAL CONTROL SEQUENCE ON AN IMMUNOCHROMATOGRAPHIC STRIP
    Publication number: 20110229887
    Abstract: Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of test and control nucleic acids. Test nucleic acids can be immobilized at multiple locations, such that amplification of either a test nucleic acid or a control nucleic acid provides a captured nucleic acid in a control capture zone.
    Type: Application
    Filed: December 7, 2010
    Publication date: September 22, 2011
    Inventors: Huimin Kong, Yanhong Tong, Wen Tang
  • Helicase-dependent amplification of nucleic acids
    Patent number: 7829284
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Grant
    Filed: April 27, 2007
    Date of Patent: November 9, 2010
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • Helicase-dependent amplification of circular nucleic acids
    Publication number: 20100075384
    Abstract: A helicase-mediated amplification method for circular DNA templates and target DNA sequences within the templates is provided. The method combines a DNA polymerase and a helicase preparation to amplify a target sequence as well as the entire circular DNA template.
    Type: Application
    Filed: March 23, 2005
    Publication date: March 25, 2010
    Applicant: New England Biolabs, Inc.
    Inventors: Huimin Kong, Yan Xu
  • Helicase-dependent amplification of RNA
    Patent number: 7662594
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Grant
    Filed: January 5, 2006
    Date of Patent: February 16, 2010
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • Helicase-dependent amplification of nucleic acids
    Publication number: 20070254304
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Application
    Filed: April 25, 2007
    Publication date: November 1, 2007
    Applicant: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • Helicase dependent amplification of nucleic acids
    Patent number: 7282328
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Grant
    Filed: September 19, 2003
    Date of Patent: October 16, 2007
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • Helicase-dependent amplification of nucleic acids
    Publication number: 20070207495
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Application
    Filed: April 27, 2007
    Publication date: September 6, 2007
    Applicant: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • Helicase-dependent amplification of nucleic acids
    Publication number: 20060154286
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Application
    Filed: January 5, 2006
    Publication date: July 13, 2006
    Applicant: New England Biolabs, Inc.
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu, Wen Tang
  • Helicase dependent amplification of nucleic acids
    Publication number: 20040058378
    Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
    Type: Application
    Filed: September 19, 2003
    Publication date: March 25, 2004
    Inventors: Huimin Kong, Myriam Vincent, Yan Xu
  • N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
    Publication number: 20030211506
    Abstract: The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.
    Type: Application
    Filed: November 14, 2002
    Publication date: November 13, 2003
    Inventors: Huimin Kong, Lauren Sears Higgins, Michael A Dalton, Rebecca B Kucera, Ira Schildkraut, Geoffrey G Wilson
  • Engineering nicking endonucleases from type IIs restriction endonucleases
    Patent number: 6395523
    Abstract: The present invention relates to methods to engineer nicking endonucleases from existing Type IIs restriction endonucleases, and the production of the engineered nicking endonucleases. Two engineering methods are disclosed. One involves inactivating the dimerization function of a Type IIs restriction enzyme using site-directed mutagenesis approach. The other involves replacing the cleavage domain of a Type IIs restriction enzyme with the cleavage domain from a natural occurring nicking endonuclease, N.BstNBI.
    Type: Grant
    Filed: June 1, 2001
    Date of Patent: May 28, 2002
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Caroline Besnier, Yan Xu
  • Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli
    Patent number: 6395531
    Abstract: The present invention relates to recombinant DNA which encodes the MlyI restriction endonuclease as well as the MlyI and BstNBII methyltransferases and expression of MlyI restriction endonuclease and M.BStNBII in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: March 21, 2001
    Date of Patent: May 28, 2002
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren Sears Higgins
  • Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli
    Patent number: 6391608
    Abstract: The present invention relates to recombinant DNA which encodes the PleI restriction endonuclease as well as PleI and BstNBII methyltransferase and expression of PleI restriction endonuclease and M.BstNBII in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: March 21, 2001
    Date of Patent: May 21, 2002
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren Sears Higgins
  • Method for cloning and producing the SwaI restriction endonuclease
    Patent number: 6245545
    Abstract: The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
    Type: Grant
    Filed: April 27, 1999
    Date of Patent: June 12, 2001
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren S. Higgins, Michael A. Dalton