Patents by Inventor Huirong Gao
Huirong Gao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200157554Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: January 30, 2020Publication date: May 21, 2020Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20190040372Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: October 18, 2018Publication date: February 7, 2019Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
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Publication number: 20190040405Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: September 13, 2018Publication date: February 7, 2019Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20190032070Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.Type: ApplicationFiled: January 25, 2017Publication date: January 31, 2019Applicant: Pioneer HIi-Bred International, Inc.Inventors: Andrew Mark CIGAN, Mark Jacob GADLAGE, Huirong GAO, Robert B MEELEY, Joshua K YOUNG
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Publication number: 20180371479Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: August 3, 2018Publication date: December 27, 2018Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young
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Patent number: 10150956Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: GrantFiled: January 10, 2018Date of Patent: December 11, 2018Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED IINTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
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Publication number: 20180305706Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: ApplicationFiled: June 13, 2018Publication date: October 25, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
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Publication number: 20180298396Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 23, 2018Publication date: October 18, 2018Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL W. LASSNER, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK A. LYZNIK, DAVID J. PETERSON, CHRISTOPHER JAY SCELONGE
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Publication number: 20180298397Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 23, 2018Publication date: October 18, 2018Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL W. LASSNER, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK A. LYZNIK, DAVID J. PETERSON, CHRISTOPHER JAY SCELONGE
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Publication number: 20180230476Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.Type: ApplicationFiled: August 31, 2015Publication date: August 16, 2018Inventors: ANDREW MARK CIGAN, HUIRONG GAO, ZHAN-BIN LIU, JASDEEP MUTTI, DEAN PODLICH, CHRISTOPHER JAY SCELONGE
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Patent number: 10030245Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: March 22, 2012Date of Patent: July 24, 2018Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Michael Lassner, Derek Jantz, James Jefferson Smith, Mark Cigan, Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev
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Publication number: 20180142223Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: January 10, 2018Publication date: May 24, 2018Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES J ENGLISH, SAVERIO CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA S ONG, SERGEI SVITASHEV, JOSHUA K YOUNG
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Patent number: 9951346Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: July 18, 2012Date of Patent: April 24, 2018Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael W. Lassner, Zhongsen Li, Zhan-Bin Liu, Leszek A. Lyznik, David J. Peterson, Christopher Jay Scelonge
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Patent number: 9909110Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: GrantFiled: October 11, 2016Date of Patent: March 6, 2018Assignees: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea Ong, Sergei Svitashev, Joshua K Young
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Publication number: 20170183677Abstract: Compositions and methods are provided for agronomic trait modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within a genomic region of a plant, plant cell or seed to provide improvement in a desirable agronomic trait such as drought, yield, and stress tolerance. Breeding methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.Type: ApplicationFiled: July 13, 2015Publication date: June 29, 2017Inventors: HUIRONG GAO, XIAOMU NIU, JINRUI SHI
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Publication number: 20170058295Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.Type: ApplicationFiled: October 11, 2016Publication date: March 2, 2017Inventors: DENNIS L. BIDNEY, Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Derek Jantz, Michael Lassner, Keith S. Lowe, Leszek Aleksander Lyznik, James Jefferson Smith
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Patent number: 9574208Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.Type: GrantFiled: June 19, 2012Date of Patent: February 21, 2017Assignees: EI DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL INCInventors: Dennis L. Bidney, Mark Cigan, Carl Falco, Huirong Gao, Derek Jantz, Mike Lassner, Keith Lowe, Leszek Aleksander Lyznik, James Jefferson Smith
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Publication number: 20170022488Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: October 11, 2016Publication date: January 26, 2017Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES ENGLISH, CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA ONG, SERGEI SVITASHEV, JOSHUA K YOUNG
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Patent number: 9499837Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.Type: GrantFiled: June 19, 2012Date of Patent: November 22, 2016Assignees: E.I. du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.Inventors: Dennis Bidney, Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Derek Jantz, Mike Lassner, Keith Lowe, Leszek A Lyznik, James Jefferson Smith
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Patent number: 9499827Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: GrantFiled: May 3, 2013Date of Patent: November 22, 2016Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea Ong, Sergei Svitashev, Joshua K Young