Abstract: A method of direct construction and isolation of full-length target cDNA molecules obtains a large number of full-length target cDNA clones with minimum screening. This new method saves time and resources that are needed in traditional methods requiring large screenings of clones that do not contain the desired sequence. A characteristic of this method includes using a total RNA population to purify mRNA for synthesizing a mixed population of first strand cDNA. Another characteristic is the use of a short sequence of the target sequence as a forward primer for the generation of the second strand cDNAs and then separating out all non-hybridizing single stranded cDNAs. Only the double stranded cDNAs are then cloned and sequenced. In this manner, only target cDNA clones are obtained and a large amount of screening is avoided.