Abstract: Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.

Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.

Abstract: A two-channel burner includes a pulverized coal supply mechanism, a transition channel, an inner secondary air guide tube, an outer secondary air guide tube, a combustion stabilizing chamber, and a flow smoothing chamber. The outer secondary air guide tube, the combustion stabilizing chamber, and the flow smoothing chamber are sequentially connected to form a burner body. The pulverized coal supply mechanism passes through an interior of the burner body. The transition channel is fitted over the pulverized coal supply mechanism. The inner secondary air guide tube is disposed between the transition channel and the outer secondary air guide tube and forms an inner secondary air passage together with the transition channel, and forms an outer secondary air passage together with the outer secondary air guide tube. An outlet end of the inner secondary air guide tube is formed to have a flared opening.