Abstract: Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

Abstract: Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

Abstract: Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

Abstract: Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

Abstract: A biochip with a radio frequency (RF) identification (ID) chip attached thereto, a hybridization system, and a hybridization management method are provided. Probes used for analyzing living body molecules are highly integrated on a substrate of the biochip, and biochip information including information obtained in the process of combining the living body molecules with the probes of the biochip is recorded in the RF ID tag of the biochip. Accordingly, it is possible to efficiently manage the biochip information using the RF ID tag and to prevent the biochip from being reused.

Abstract: Provided is a microarray holding device. The microarray holding device includes: a solid substrate in the form of a plate in which an engaging-receiving portion for receiving a microarray substrate to be engaged therewith is formed, the engaging-receiving portion being composed of an opening; sidewalls to be aligned with sidewalls of the microarray substrate to be engaged with the engaging-receiving portion; and a base surface on which a top or bottom surface of the microarray substrate to be engaged with the engaging-receiving portion is to be placed, wherein the base surface is depressed from a top or bottom surface of the solid substrate such that the other surface of the microarray substrate is lower than the top or bottom surface of the solid substrate when the microarray substrate is engaged with the engaging-receiving portion.

Abstract: A spotting device for manufacturing a DNA microarray and a spotting method using the same are provided.

Abstract: Provided is a patch for a reaction chamber of a microarray, which includes a support means provided with a sample inlet and an adhesive means adhering to the support means and providing an adhesive strength capable of being attached to a microarray substrate, wherein the adhesive means has stacked adhesive means supports having both surfaces to which an adhesive material is deposited and a first adhesive material located between the adhesive means support and the adhesive means support or between the adhesive means support and the support means has 33 oz/in greater adhesive strength (according to ASTM D3330 Method), based on an adhesive strength to steel, than a second adhesive material located between the patch and the microarray substrate.