Patents by Inventor Hung-Chi Chien
Hung-Chi Chien has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11920190Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.Type: GrantFiled: December 28, 2020Date of Patent: March 5, 2024Assignee: Industrial Technology Research InstituteInventors: Pei-Shin Jiang, Jenn-Yeh Fann, Hung-Chi Chien, Yu-Yu Lin, Chih-Lung Lin
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Patent number: 11676682Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: November 1, 2019Date of Patent: June 13, 2023Assignee: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Patent number: 11311592Abstract: Disclosed herein is the novel use of use of a Bauhinia spp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus the Bauhinia spp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.Type: GrantFiled: February 24, 2020Date of Patent: April 26, 2022Assignees: ACADEMIA SINICA, INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chang-Jen Huang, Rita P.-Y. Chen, Yung-Feng Liao, Bo-Kai Wu, Po-Ting Chen, Hui-Ping Tsai, Yi-Cheng Cheng, Cheng-Li Fang, Hung-Chi Chien, Tien-Soung Tong, Ting-Shou Chen, Shu-Jiau Chiou
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Publication number: 20210214780Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.Type: ApplicationFiled: December 28, 2020Publication date: July 15, 2021Applicant: Industrial Technology Research InstituteInventors: Pei-Shin Jiang, Jenn-Yeh Fann, Hung-Chi Chien, Yu-Yu Lin, Chih-Lung Lin
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Publication number: 20200246410Abstract: Disclosed herein is the novel use of use of a Bauhinia spp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus the Bauhinia spp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.Type: ApplicationFiled: February 24, 2020Publication date: August 6, 2020Inventors: Chang-Jen HUANG, Rita P.-Y. CHEN, Yung-Feng LIAO, Bo-Kai WU, Po-Ting CHEN, Hui-Ping TSAI, Yi-Cheng CHENG, Cheng-Li FANG, Hung-Chi CHIEN, Tien-Soung TONG, Ting-Shou CHEN, Shu-Jiau CHIOU
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Patent number: 10610557Abstract: Disclosed herein is the novel use of use of a Bauhinia spp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus the Bauhinia spp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.Type: GrantFiled: November 3, 2016Date of Patent: April 7, 2020Assignees: ACADEMIA SINICA, INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chang-Jen Huang, Rita P.-Y. Chen, Yung-Feng Liao, Bo-Kai Wu, Po-Ting Chen, Hui-Ping Tsai, Yi-Cheng Cheng, Cheng-Li Fang, Hung-Chi Chien, Tien-Soung Tong, Ting-Shou Chen, Shu-Jiau Chiou
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Patent number: 10515714Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: July 25, 2017Date of Patent: December 24, 2019Assignee: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Publication number: 20180318371Abstract: Disclosed herein is the novel use of use of a Bauhinia spp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus the Bauhinia spp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.Type: ApplicationFiled: November 3, 2016Publication date: November 8, 2018Inventors: Chang-Jen HUANG, Rita P.-Y. CHEN, Yung-Feng LIAO, Bo-Kai WU, Po-Ting CHEN, Hui-Ping TSAI, Yi-Cheng CHENG, Cheng-Li FANG, Hung-Chi CHIEN, Tien-Soung TONG, Ting-Shou CHEN, Shu-Jiau CHIOU
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Publication number: 20180018425Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: ApplicationFiled: July 25, 2017Publication date: January 18, 2018Applicant: Industrial Technology Research InstituteInventors: Chao-Chi PAN, Jenn-Yeh FANN, Chung-Fan CHIOU, Hung-Chi CHIEN, Hui-Ling CHEN
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Patent number: 9777321Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.Type: GrantFiled: September 9, 2016Date of Patent: October 3, 2017Assignee: Industrial Technology Research InstituteInventors: Chung-Fan Chiou, Ying-Chih Pu, Chao-Chi Pan, Chih-Tsung Shih, Ming-Chia Li, Chein-Shiu Kuo, Hung-Chi Chien, Chang-Sheng Chu
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Patent number: 9767251Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: May 20, 2015Date of Patent: September 19, 2017Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Patent number: 9747414Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: May 23, 2014Date of Patent: August 29, 2017Assignee: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Publication number: 20170009286Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.Type: ApplicationFiled: September 9, 2016Publication date: January 12, 2017Inventors: Chung-Fan Chiou, Ying-Chih Pu, Chao-Chi Pan, Chih-Tsung Shih, Ming-Chia Li, Chein-Shiu Kuo, Hung-Chi Chien, Chang-Sheng Chu
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Patent number: 9482615Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.Type: GrantFiled: March 11, 2011Date of Patent: November 1, 2016Assignee: Industrial Technology Research InstituteInventors: Chung-Fan Chiou, Ying-Chih Pu, Chao-Chi Pan, Chih-Tsung Shih, Ming-Chia Li, Chein-Shiu Kuo, Hung-Chi Chien, Chang-Sheng Chu
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Publication number: 20150379194Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: ApplicationFiled: May 20, 2015Publication date: December 31, 2015Applicant: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Publication number: 20140256570Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: ApplicationFiled: May 23, 2014Publication date: September 11, 2014Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Patent number: 8673559Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: June 8, 2012Date of Patent: March 18, 2014Assignee: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Publication number: 20130230909Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: ApplicationFiled: May 7, 2013Publication date: September 5, 2013Applicant: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Patent number: 8486630Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: GrantFiled: November 5, 2009Date of Patent: July 16, 2013Assignee: Industrial Technology Research InstituteInventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen
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Publication number: 20120295260Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.Type: ApplicationFiled: June 8, 2012Publication date: November 22, 2012Inventors: Chao-Chi Pan, Jenn-Yeh Fann, Chung-Fan Chiou, Hung-Chi Chien, Hui-Ling Chen