Abstract: The disclosure relates to cyclopropyl derivatives and methods of use. In some embodiments, the disclosure relates to methods of managing medical disorders with pharmaceutical compositions disclosed herein administered to subject in need thereof. In certain embodiments, the disclosure relates to methods of managing mental disorders, mood disorders, pain, and fibromyalgia and related conditions with pharmaceutical compositions disclosed herein.

Abstract: The disclosure relates to cyclopropyl derivatives and methods of use. In some embodiments, the disclosure relates to methods of managing medical disorders with pharmaceutical compositions disclosed herein administered to subject in need thereof. In certain embodiments, the disclosure relates to methods of managing mental disorders, mood disorders, pain, and fibromyalgia and related conditions with pharmaceutical compositions disclosed herein.

Abstract: This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications.

Abstract: This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.

Abstract: By this invention, further properties and uses of medium-chain thioesterases in plants are provided, including methods of using medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells.

Abstract: This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.

Abstract: This invention relates to a novel class of plant enzymes, thioesterases having preferential hydrolase activity toward one or more medium-chain acyl-ACP substrates. Purification, especially the removal of substantially all other plant proteins, and use of the plant thioesterase is provided, including the use of the protein as a tool in gene isolation for biotechnological applications and a description of various uses for such nucleic acid sequences thereto. The enzyme is preferrably obtained from Umbellularia californica and has preferential hydrolase activity toward C12 acyl-ACP substrate.

Abstract: This invention relates to a novel class of plant enzymes, thioesterases having preferential hydrolase activity toward one or more medium-chain acyl-ACP substrates. Purification, especially the removal of substantially all other plant proteins, and use of the plant thioesterase is provided, including the use of the protein as a tool in gene isolation for biotechnological applications and a description of various uses for such nucleic acid sequences thereto.

Abstract: A method and assay kit for determining the presence of an enzyme in a sample are provided. A sample suspected of containing the enzyme is applied to an image gel, which may be any conventional material, typically agarose gel. The sample may first be subjected to electrophoresis, or may be detected directly using the present invention. The image gel includes an immobilized phase capable of binding a product of the enzyme but not the substrate. By exposing the enzyme to substrate, and drawing the resulting reaction mixtures through the image gel, only the product is bound in the image gel. The presence of enzyme may then be determined by detecting the product in the image gel. In addition to developing electrophoresis gels, the method of the present invention will find great use in screening a plurality of complex mixtures which have been separated by other conventional techniques.