Patents by Inventor Hwei-In Shawn LIM

Hwei-In Shawn LIM has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Differentiation of pluripotent cells into microglial cells expressing IBA-1
    Patent number: 11618886
    Abstract: The present invention relates to a method of culturing primitive macrophages from stem cells. Specifically, the method comprises contacting and incubating stem cells with a serum-free culture media comprising a GSK3 inhibitor to differentiate stem cells into cell of the mesoderm lineage, contacting and incubating cells of the mesoderm lineage with a culture media comprising DKK1 to differentiate the cells into the hematopoietic lineage, maturing the cells of the hematopoietic lineage and contacting and incubating these cells with a culture media comprising M-CSF to drive differentiation into primitive-like macrophages. The invention also relates to a primitive-like macrophage, use of the primitive-like macrophage and a kit when used in the method of the invention.
    Type: Grant
    Filed: June 29, 2020
    Date of Patent: April 4, 2023
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Hwei-In Shawn Lim, Tara Huber, Florent Ginhoux
  • DIFFERENTIATION OF MACROPHAGES FROM PLURIPOTENT STEM CELLS
    Publication number: 20200399601
    Abstract: The present invention relates to a method of culturing primitive macrophages from stem cells. Specifically, the method comprises contacting and incubating stem cells with a serum-free culture media comprising a GSK3 inhibitor to differentiate stem cells into cell of the mesoderm lineage, contacting and incubating cells of the mesoderm lineage with a culture media comprising DKK1 to differentiate the cells into the hematopoietic lineage, maturing the cells of the hematopoietic lineage and contacting and incubating these cells with a culture media comprising M-CSF to drive differentiation into primitive-like macrophages. The invention also relates to a primitive-like macrophage, use of the primitive-like macrophage and a kit when used in the method of the invention.
    Type: Application
    Filed: June 29, 2020
    Publication date: December 24, 2020
    Inventors: Hwei-In Shawn LIM, Tara Huber, Florent Ginhoux
  • Method of differentiating human pluripotent stem cells into primitive macrophages
    Patent number: 10724003
    Abstract: The present invention relates to a method of culturing primitive-like macrophages from stem cells, a kit when used in the method thereof and uses of the primitive like macrophage for in-vitro disease models and for screening compounds for therapy. One embodied culture method comprises contacting and incubating embryonic stem cells or induced pluripotent stem cells with a serum-free culture media comprising a GSK3 inhibitor to differentiate stem cells into cells of the mesoderm lineage, followed by incubation with a culture media comprising Dickkopf-related protein 1 (DKK1) to differentiate the mesoderm into cells of hematopoietic lineage, maturing hematopoietic cells and incubating these cells with a culture media comprising M-CSF to drive differentiation into primitive-like macrophages.
    Type: Grant
    Filed: January 18, 2016
    Date of Patent: July 28, 2020
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Hwei-In Shawn Lim, Tara Huber, Florent Ginhoux
  • DIFFERENTIATION OF MACROPHAGES FROM PLURIPOTENT STEM CELLS
    Publication number: 20180010096
    Abstract: The present invention relates to a method of culturing primitive-like macrophages from stem cells, a kit when used in the method thereof and uses of the primitive like macrophage for in-vitro disease models and for screening compounds for therapy. One embodied culture method comprises contacting and incubating embryonic stem cells or induced pluripotent stem cells with a serum-free culture media comprising a GSK3 inhibitor to differentiate stem cells into cells of the mesoderm lineage, followed by incubation with a culture media comprising Dickkopf-related protein 1 (DKK1) to differentiate the mesoderm into cells of hematopoietic lineage, maturing hematopoietic cells and incubating these cells with a culture media comprising M-CSF to drive differentiation into primitive-like macrophages.
    Type: Application
    Filed: January 18, 2016
    Publication date: January 11, 2018
    Inventors: Hwei-In Shawn LIM, Tara HUBER, Florent GINHOUX