Patents by Inventor Hwei-Ling Shen

Hwei-Ling Shen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Application of the broccoli wound-inducible promoter of gene in transgenic plants
    Patent number: 9085768
    Abstract: In this study, we used a wound-inducible promoter of the broccoli (Brassica oleracea var. italica) GLUCOSE INHIBITION of ROOT ELONGATION1 (GIR1) gene fused to ?-glucuronidase (GUS, pBoGIR1::GUS) as a selectable marker. Transgenic broccoli plants expressing pBoGIR1::GUS appear blue in planta at wounded regions after GUS staining for 30 min. Similarly, the blue color is visible in transgenic Arabidopsis and rice plants expressing pBoGIR1::GUS at wounded areas after GUS staining for 2 h, indicating that this promoter is wound-inducible in both dicots and monocots. GUS staining is very rapid and the partial wounding in this study is a nondestructive method that does not affect further plant growth and development. Thus, pBoGIR1::GUS could serve as an effective substitute for antibiotic- and herbicide-resistance genes in the generation of genetically modified crops.
    Type: Grant
    Filed: August 10, 2012
    Date of Patent: July 21, 2015
    Assignee: Academia Sinica
    Inventors: Wan-Hsing Cheng, Ming-Hau Chiang, Ya-Huei Chen, Hwei-Ling Shen
  • Application of the broccoli wound-inducible promoter of GLUCOSE INHIBITION of ROOT ELONGATION 1 gene in transgenic plants
    Publication number: 20140013472
    Abstract: In this study, we used a wound-inducible promoter of the broccoli (Brassica oleracea var. italica) GLUCOSE INHIBITION of ROOT ELONGATION1 (GIR1) gene fused to ?-glucuronidase (GUS, pBoGIR1::GUS) as a selectable marker. Transgenic broccoli plants expressing pBoGIR1::GUS appear blue in planta at wounded regions after GUS staining for 30 min. Similarly, the blue color is visible in transgenic Arabidopsis and rice plants expressing pBoGIR1::GUS at wounded areas after GUS staining for 2 h, indicating that this promoter is wound-inducible in both dicots and monocots. GUS staining is very rapid and the partial wounding in this study is a nondestructive method that does not affect further plant growth and development. Thus, pBoGIR1::GUS could serve as an effective substitute for antibiotic- and herbicide-resistance genes in the generation of genetically modified crops.
    Type: Application
    Filed: August 10, 2012
    Publication date: January 9, 2014
    Applicant: Academia Sinica
    Inventors: Wan-Hsing Cheng, Ming-Hau Chiang, Ya-Huei Chen, Hwei-Ling Shen