Abstract: The present invention may provide a cell culture device and a cell culture system comprising same, wherein the cell culture device comprises: a plurality of upper chambers provided with a porous membrane on the lower side thereof and first accommodation spaces thereinside; a lower chamber configured to provide a second accommodation spaces therein; and a support configured to prevent the plurality of upper chambers from coming into contact with the lower chamber and configured such that the plurality of upper chambers are spaced apart from each other in a horizontal direction by a predetermined distance, wherein the first accommodation spaces and the second accommodation spaces are configured to exchange materials with each other through the porous membrane, and the second accommodation spaces have a material exchange area common to each of the first accommodation spaces provided in the plurality of upper chambers.

Abstract: The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.