Abstract: An optical gene biosensor is disclosed. The optical gene biosensor includes a substrate; a molecular beacon anchored to the substrate, wherein the molecular beacon includes an oligonucleotide specifically binding to a target nucleic acid molecule and a first compound bound to a first terminal of the oligonucleotide; an optical marker specifically binding to the first compound, wherein the optical marker is configured to retro-reflect irradiated light; a light source for irradiating the optical marker with light; and a light-receiver for receiving light retro-reflected from the optical marker. The optical gene biosensor may perform accurate quantitative analysis of a target nucleic acid molecule using both non-spectral and spectral light sources.

Abstract: A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.

Abstract: An optical bio-sensing device includes a transparent substrate covering a top of a space accommodating therein a sample containing a target bio-material; a signal converter fixed to the transparent substrate, and including the upconversion nanoparticles for receiving incident light and emitting converted light of a wavelength shorter than a wavelength of the incident light; a signal reflector including retroreflection particles bindable to the signal converter via the target bio-material, wherein the retroreflection particles retroreflect the converted light; a light source for irradiating the incident light to the signal converter; and a light receiver for receiving light retroreflected from the signal reflector.

Abstract: An optical gene biosensor is disclosed. The optical gene biosensor includes a substrate; a molecular beacon anchored to the substrate, wherein the molecular beacon includes an oligonucleotide specifically binding to a target nucleic acid molecule and a first compound bound to a first terminal of the oligonucleotide; an optical marker specifically binding to the first compound, wherein the optical marker is configured to retro-reflect irradiated light; a light source for irradiating the optical marker with light; and a light-receiver for receiving light retro-reflected from the optical marker. The optical gene biosensor may perform accurate quantitative analysis of a target nucleic acid molecule using both non-spectral and spectral light sources.

Abstract: Disclosed is a method of manufacturing lanthanide fluorescent silica nanoparticles, including a complex synthesis step of synthesizing a silane-lanthanide chelate complex, an emulsion formation step of forming a water-in-oil microemulsion by dispersing micelles, the water-phase core of which is introduced with the silane-lanthanide chelate complex, in an oil-phase solvent, a silica introduction step of introducing a silica precursor into the microemulsion, and a nanoparticle synthesis step of synthesizing fluorescent silica nanoparticles by crosslinking the silica precursor and the silane-lanthanide chelate complex in the micelles. The lanthanide fluorescent silica nanoparticles thus manufactured can be utilized in fluorescence analysis of inorganic materials or bio-derived materials.