Abstract: A service identifying and processing method using a wireless terminal message according to an exemplary embodiment of the present invention includes (a) receiving a wireless terminal message by a first entity which is a mobile device; and (b) expressing, by a first agent which is an information processing application program installed on the first entity, entity information of second entity based on the wireless terminal message and service confirmation information related to service provided by the second entity, through an application screen by the first agent.

Abstract: Provided is an electrochemical aptasensor for detecting di(2-ethylhexyl)phthalate (DEHP) with high sensitivity. The electrochemical aptasensor according to the present invention has a low detection limit concentration by improving sensitivity by sensor surface modification using a nano composite and gold nanoflowers, and has high practical applicability of a sensor by monitoring a trace amount of DEHP migrating from a real plastic product by a simple measurement method.

Abstract: The present invention relates to a microfluidic mixer and a microfluidic device including the same, and in the microfluidic mixer according to the present invention, a disk-shaped mixing unit with double U-shaped protruding portions formed therein can be continuously provided along a microchannel, thereby increasing collisions of samples to improve the binding efficiency thereof and shorten the binding time. Furthermore, the microfluidic device according to the present invention can detect a target material at high speed even at a high flow rate by including the microfluidic mixer, and thus can be usefully utilized for early diagnosis and prognosis diagnosis of a disease such as cancer.

Abstract: The present invention relates to an electrochemical biosensor for detecting a target RNA, and the present invention can detect a very small amount of target RNA with high sensitivity without a nucleic acid amplification reaction through a CRISPR/Cas13a trans-cleavage reaction, thereby having an advantage of being useful for point-of-care diagnostic testing of fast-spreading RNA-based infectious diseases such as COVID-19.

Abstract: Provided is an electrochemical aptasensor for detecting di(2-ethylhexyl)phthalate (DEHP) with high sensitivity. The electrochemical aptasensor according to the present invention has a low detection limit concentration by improving sensitivity by sensor surface modification using a nano composite and gold nanoflowers, and has high practical applicability of a sensor by monitoring a trace amount of DEHP migrating from a real plastic product by a simple measurement method.

Abstract: The present invention relates to a microfluidic mixer and a microfluidic device including the same, and in the microfluidic mixer according to the present invention, a disk-shaped mixing unit with double U-shaped protruding portions formed therein can be continuously provided along a microchannel, thereby increasing collisions of samples to improve the binding efficiency thereof and shorten the binding time. Furthermore, the microfluidic device according to the present invention can detect a target material at high speed even at a high flow rate by including the microfluidic mixer, and thus can be usefully utilized for early diagnosis and prognosis diagnosis of a disease such as cancer.

Abstract: The present invention relates to a microfluidic separation device, a separation method using the same and a kit for separating circulating rare cells from blood using the same, and more particularly, to a microfluidic-based separation technology for fixing target particles of a sample, which have a specific affinity for magnetic nanoparticles, to a device by use of a magnetic material, and for isolating the sample from which the target particles have been removed. The present invention may be effectively applied to remove leukocytes from a blood sample in order to isolate circulating rare cells (CRCs), particularly circulating tumor cells (CTCs).

Abstract: A particle separating apparatus and method are provided, which pass a fluid sample such as blood through a filter to remove foreign matter, and separate target particles by using a MOFF channel, and re-separate the separated target particles through dielectrophoresis. The particle separating apparatus includes a MOFF (Multi Orifice Flow Fractionation) channel including a multi orifice segment through which a fluid sample passes to discharge a primarily separated material that are target particles separated from the fluid sample, through a central passage; a dielectrophoresis channel including a pair of electrodes to which AC power is applied and forming an electric field in a flow channel connected to the central passage of the MOFF channel to re-separate the target particles from the primarily separated material discharged from the central passage of the MOFF channel through dielectrophoresis.

Abstract: The present invention relates to a microfluidic separation device, a separation method using the same and a kit for separating circulating rare cells from blood using the same, and more particularly, to a microfluidic-based separation technology for fixing target particles of a sample, which have a specific affinity for magnetic nanoparticles, to a device by use of a magnetic material, and for isolating the sample from which the target particles have been removed. The present invention may be effectively applied to remove leukocytes from a blood sample in order to isolate circulating rare cells (CRCs), particularly circulating tumor cells (OTCs).

Abstract: A particle separating apparatus and method are provided, which pass a fluid sample such as blood through a filter to remove foreign matter, and separate target particles by using a MOFF channel, and re-separate the separated target particles through dielectrophoresis. The particle separating apparatus includes a MOFF (Multi Orifice Flow Fractionation) channel including a multi orifice segment through which a fluid sample passes to discharge a primarily separated material that are target particles separated from the fluid sample, through a central passage; a dielectrophoresis channel including a pair of electrodes to which AC power is applied and forming an electric field in a flow channel connected to the central passage of the MOFF channel to re-separate the target particles from the primarily separated material discharged from the central passage of the MOFF channel through dielectrophoresis.

Abstract: A device separating target particles in a fluid sample includes first through third multi-orifice flow fractionation (“MOFF”) channels, each including a multi-orifice segment with an inlet and an outlet at opposite ends, and an alternating series of contraction channels and expansion chambers interconnected in a lengthwise direction; a first separation unit including a first separation channel which is interconnected in fluid communication with a center region of the outlet of the first MOFF channel, and first branch channels which are interconnected in fluid communication with sidewall regions of the outlet of the first MOFF channel, and respectively with inlets of the second and third MOFF channels; and buffer inlets which are connected to the inlets of the second and third MOFF channels and through which a buffer flows into the second and third MOFF channels.

Abstract: A device for separating micro particles is provided. The separating device comprises a sample inlet into which a sample containing micro particles is injected; fluid inlets into which fluid is injected to form a flow sheath for the sample; a plurality of outlets through which the micro particles are separated and discharged out; a channel through which the sample and the fluid flow; and a first electrode and a second electrode longitudinally disposed in parallel in the channel. The first and the second electrodes are provided in such a manner that an electrode gap between the first and the second electrodes has a curved shape. The micro particles in the sample are easily separated using a dielectrophoresis characteristic.

Abstract: A device separating target particles in a fluid sample includes first through third multi-orifice flow fractionation (“MOFF”) channels, each including a multi-orifice segment with an inlet and an outlet at opposite ends, and an alternating series of contraction channels and expansion chambers interconnected in a lengthwise direction; a first separation unit including a first separation channel which is interconnected in fluid communication with a center region of the outlet of the first MOFF channel, and first branch channels which are interconnected in fluid communication with sidewall regions of the outlet of the first MOFF channel, and respectively with inlets of the second and third MOFF channels; and buffer inlets which are connected to the inlets of the second and third MOFF channels and through which a buffer flows into the second and third MOFF channels.

Abstract: A device for detecting micro particles in gas, which comprises: an inlet through which a gaseous sample including micro particles flows in; an outlet through which the sample flows out; a channel through which the sample flows from the inlet toward the outlet; a cooling layer which cools and condenses the sample flowing in the channel; a reservoir which is positioned on the cooling layer and collects the condensed sample; a detector which is positioned in the reservoir on the cooling layer and detects the micro particles included in the collected sample; and a heater which heats the outlet portion to produce a pressure difference between the inlet portion and the outlet portion, so that the sample flows through the channel from the inlet toward the outlet. The device for detecting micro particles in gas provides the advantage that micro particles included in gaseous sample can be detected without having to use additional pump or collector, detection time can be reduced, and detection accuracy can be improved.

Abstract: A device for detecting micro particles in gas, which comprises: an inlet through which a gaseous sample including micro particles flows in; an outlet through which the sample flows out; a channel through which the sample flows from the inlet toward the outlet; a cooling layer which cools and condenses the sample flowing in the channel; a reservoir which is positioned on the cooling layer and collects the condensed sample; a detector which is positioned in the reservoir on the cooling layer and detects the micro particles included in the collected sample; and a heater which heats the outlet portion to produce a pressure difference between the inlet portion and the outlet portion, so that the sample flows through the channel from the inlet toward the outlet. The device for detecting micro particles in gas provides the advantage that micro particles included in gaseous sample can be detected without having to use additional pump or collector, detection time can be reduced, and detection accuracy can be improved.

Abstract: A device for separating micro particles is provided. The separating device comprises a sample inlet into which a sample containing micro particles is injected; fluid inlets into which fluid is injected to form a flow sheath for the sample; a plurality of outlets through which the micro particles are separated and discharged out; a channel through which the sample and the fluid flow; and a first electrode and a second electrode longitudinally disposed in parallel in the channel. The first and the second electrodes are provided in such a manner that an electrode gap between the first and the second electrodes has a curved shape. The micro particles in the sample are easily separated using a dielectrophoresis characteristic.

Abstract: The present invention provides an elastase-inhibiting protein isolated ("Guamerin") from a Korean leech, Guameri (Hirudo nipponia) and a process for preparing the same. Guamerin is a protein of a molecular weight of 6,110 Da which is composed of 57 amino acid residues, whose active site is composed of 36-methionine and 37-isoleucine, which retains an inhibiting-activity highly specific to elastase, and which shows stability against heat as well as strong acids and alkalies. Guamerin of the present invention can be applied in the treatment of diseases associated with an excess level of elastase, such as rheumatoid arthritis, emphysema, and psoriasis.