Abstract: Disclosed are a novel Korean isolate of tomato yellow leaf curl virus (TYLCV) and infectious clones thereof, wherein: the use of an inventive recombinant vector comprising the nucleotide sequence of tomato yellow leaf curl virus (TYLCV) and Agrobacterium tumefaciens comprising the recombinant vector can effectively induce tomato yellow leaf curl virus infection in a crop even without an insect vector; an inventive infection system, compared with existing TYLCV Korean isolates, shows excellent infectivity in a plant, and thus can induce symptoms even in TYLCV-resistant varieties of tomatoes having existing widely used Ty-1 or Ty-2 resistant gene, so that the infectious system can be utilized as an artificial infection system in the future and can be applied in various studies in the future, such as a host plant range for tomato yellow leaf curl virus (TYLCV) and the correlation with the host plant.