Abstract: The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene—specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.

Abstract: The present disclosure relates to a complementary double-stranded oligo, in which, for the amplification of a particular gene sequence, an inosine linker is linked to the 5?-terminus of a primer for the corresponding sequence, a sequence complementary to the primer is linked to the inosine linker, and at least one mis-matched nucleotide is included in the complementary sequence to form a bubble structure; in which, depending on the treatment temperature, at a predetermined temperature or lower, a single stranded oligo is turned into a double-stranded form to exist in an inactivation form, and at a predetermined temperature or higher, the oligo is activated into a single-stranded oligo; and in which, a fluorescent substance (reporter dye) and a quenching material (quencher molecule) are attached to the oligo, so that the oligo can be applied as a primer or a probe, and thus only two oligos can realize the gene amplification and fluorescent signal real-time measurement with high specificity, and to a measuring

Abstract: The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene-specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.

Abstract: The present disclosure relates to a complementary double-stranded oligo, in which, for the amplification of a particular gene sequence, an inosine linker is linked to the 5?-terminus of a primer for the corresponding sequence, a sequence complementary to the primer is linked to the inosine linker, and at least one mis-matched nucleotide is included in the complementary sequence to form a bubble structure; in which, depending on the treatment temperature, at a predetermined temperature or lower, a single stranded oligo is turned into a double-stranded form to exist in an inactivation form, and at a predetermined temperature or higher, the oligo is activated into a single-stranded oligo; and in which, a fluorescent substance and a quenching material are attached to the oligo, so that the oligo can be applied as a primer or a probe, and thus only two oligos can realize the gene amplification and fluorescent signal real-time measurement with high specificity.