Abstract: The present invention relates to a non-invasive prenatal testing method and, more particularly, to a method for enhancing the sensitivity and accuracy of non-invasive prenatal testing by applying multi-dimensional threshold values based on multiple Z-scores. Designed to reduce false-positive and false-negative possibility by applying two or more Z-score threshold values to aneuploidy detection for one chromosome, the non-invasive prenatal testing method according to the present invention exhibits the effect of obtaining a more sensitive and more accurate test result. Further, the method can minimize test errors in spite of using a small number of nucleotide sequence fragments, with the resultant effect of reducing an experiment cost and thus expensive testing cost and rapidly performing testing with a low expense.