Abstract: The present disclosure relates to a method of preparing a polymer, which includes: adding an alkyl-substituted aromatic vinyl-based monomer and a vinyl cyanide-based monomer to a reactor and initiating polymerization; and performing polymerization while continuously adding an alkyl-substituted aromatic vinyl-based monomer and a multifunctional initiator to the reactor, wherein the continuous addition of the alkyl-substituted aromatic vinyl-based monomer and the multifunctional initiator is initiated when a polymerization conversion rate is 10 to 30%, and the continuous addition of the alkyl-substituted aromatic vinyl-based monomer and the multifunctional initiator is terminated when a polymerization conversion rate is 70 to 80%.

Abstract: There is provided a method of preparing a fluorescent silk fibroin solution, which includes a) obtaining scoured fluorescent silk fibroin by adding transgenic fluorescent silkworm cocoons to an aqueous solution including a scouring agent, heating the transgenic fluorescent silkworm cocoons at 40 to 60° C. for 8 to 24 hours and washing the transgenic fluorescent silkworm cocoons with distilled water, b) dissolving the scoured fluorescent silk fibroin at 40 to 60° C. for 1 to 5 hours in a solvent in which 15 mg to 1.5 g of dithiothreitol (DTT) is mixed per 100 mL of 9 to 9.6 M LiBr, and c) dialyzing the dissolved fluorescent silk fibroin in single distilled water for 48 to 96 hours. The scouring agent includes an alcalase and sodium hydrogen carbonate (NaHCO3). The conventional methods of preparing a silk fibroin solution from fluorescent silkworm cocoons have drawbacks in that silk fibroin is not dissolved at a low temperature and fluorescence is lost.