Abstract: This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.

Abstract: This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.

Abstract: Disclosed herein is a microvolume sampling device that includes a chip and a UV-transmissive cell formed of fused silica. The chip has a plurality of wells, a receiving area extending therebetween, and a port extending through the chip to the receiving area. The cell, which preferably has a rectangular cross-section, is securingly positioned within the receiving area and extends into the wells. A chamber is defined by the cell, and a fluid sample can be drawn into the chamber from at least one of the wells by capillary force. The microvolume sampling device can be disposed upon a tray having an opening such that the port of the microvolume sampling device is in alignment with the opening to define an optical path for spectroscopic measurement of the fluid sample. The tray can be provided as a well plate for receiving a plurality of microvolume sampling devices.