Abstract: A portable gas analyzer includes multiple docking stations connected to a pneumatic flowpath through the gas analyzer. Modules are removably mounted to the docking stations. Some modules can be mounted at any one of the docking stations. The modules can include gas sensors and a microcontroller configured to generate data based on signals generated by the gas sensors. The modules are configured to simultaneously form each of electrical, mechanical, and pneumatic connections with the gas analyzer when mounted to the docking station.

Abstract: Methods for selectively adding one or more reagents are provided. In certain aspects, the methods include selectively merging one or more droplets of a plurality of droplets with one or more droplets of a plurality of reagent droplets based on detection of a property. Systems, devices and kits for practicing the subject methods are also provided. The subject disclosure may find use in a wide variety of applications, such as increasing the accuracy and/or efficiency of single-cell sequencing, detection of cancer or other diseases, monitoring disease progression, analyzing the DNA or RNA content of cells, and other applications in which it is desired to detect and/or quantify specific target cells.

Abstract: This invention provides methods for near-instantaneously separating cells that have undergone RNA guided genome modifications into pre-templated instant partitions (PIPs) and using the PIPs to associate the guide RNAs with the gene expression level changes that resulted from the genome modification.

Abstract: The invention provides high-throughput systems and methods for screening CRISPR-edited cells in bulk with single cell resolution. Methods of the invention use cells expressing polyadenylated guide RNAs that are detectable by RNA sequencing. Methods of the invention provide for the detection of each cell's guide RNA along with its single cell transcriptome to provide useful gene expression data for assessing CRISPR activity from cells in bulk. In addition, methods of the invention offer a high throughput single cell analytical framework for generating single cell transcriptome data from which CRISPR activity may be evaluated.

Abstract: A cover for a support beam in a storage system includes a plate; an abutment portion extending upwards from the plate; and a guide projection formed on the upper surface of the plate and extending at an angle to the abutment portion. The plate further includes at least one positioning projection formed in a side of the plate and extending along the guide projection.

Abstract: A support component for a storage cabinet includes an elongate bar; and a resilient leg formed in the upper surface of the bar and extending between two opposite sides of the bar. The resilient leg includes an engagement protrusion.

Abstract: The present disclosure provides ultrahigh-throughput single cell genomic sequencing methods, referred to herein as “SiC-seq”, which methods include encapsulating single cells in molten gel droplets to facilitate bulk cell lysis and purification of genomic DNA in microgels. Systems and devices for practicing the subject methods are also provided.

Abstract: The methods described herein provide an improved approach for generating monodispersed droplets. Monodispersed droplets may be effectively obtained by using a plurality of particles to trigger the breakup of a jet, which can include, e.g., flowing in a channel of a microfluidic device a first fluid into a second fluid under stable jetting conditions to provide a jet of the first fluid in the second fluid, wherein the first fluid is immiscible with the second fluid; and introducing a plurality of particles into the jet of the first fluid triggering break-up of the jet of the first fluid and encapsulation of the plurality of particles in a plurality of monodispersed droplets of the first fluid in the second fluid.

Abstract: A shelf system (1) comprises: a frame (5) for forming at least one shelf (70), and a plurality of primary and secondary rails (40, 50). The frame comprises a plurality of mounting portions (20, 30), to each of which at least one of a primary rail (40) and a secondary rail (50) is removably mountable to form lanes of different widths. Each lane includes a primary rail on one side and a secondary rail on the other side for supporting and guiding at least one item (60) in the lane, and the primary rails each comprise at least one sensor (700) for sensing the item (60) in the lane.

Abstract: A drawer (100) comprising at least one receptacle (103) open at the top of the drawer; a drawer information means extending in a depth direction of the drawer, wherein the drawer information means comprises a series of projections (108) moulded into the drawer. The drawer also comprises guiding slots (104) for holding an insert (400) having a plate (401); and a pair of legs (404a, 404b) formed in the body of the plate and extending longitudinally upwards along the sides of the plate.

Abstract: The present disclosure provides ultrahigh-throughput single cell genomic sequencing methods, referred to herein as “SiC-seq”, which methods include encapsulating single cells in molten gel droplets to facilitate bulk cell lysis and purification of genomic DNA in microgels. Systems and devices for practicing the subject methods are also provided.

Abstract: A cover for a support beam in a storage system includes a plate; an abutment portion extending upwards from the plate; and a guide projection formed on the upper surface of the plate and extending at an angle to the abutment portion. The plate further includes at least one positioning projection formed in a side of the plate and extending along the guide projection.

Abstract: A support component for a storage cabinet includes an elongate bar; and a resilient leg formed in the upper surface of the bar and extending between two opposite sides of the bar. The resilient leg includes an engagement protrusion.

Abstract: A drawer (100) comprising at least one receptacle (103) open at the top of the drawer; a drawer information means extending in a depth direction of the drawer, wherein the drawer information means comprises a series of projections (108) moulded into the drawer. The drawer also comprises guiding slots (104) for holding an insert (400) having a plate (401); and a pair of legs (404a, 404b) formed in the body of the plate and extending longitudinally upwards along the sides of the plate.

Abstract: The present disclosure provides ultrahigh-throughput single cell genomic sequencing methods, referred to herein as “SiC-seq”, which methods include encapsulating single cells in molten gel droplets to facilitate bulk cell lysis and purification of genomic DNA in microgels. Systems and devices for practicing the subject methods are also provided.

Abstract: A shelf system (1) comprises: a frame (5) for forming at least one shelf (70), and a plurality of primary and secondary rails (40, 50). The frame comprises a plurality of mounting portions (20, 30), to each of which at least one of a primary rail (40) and a secondary rail (50) is removably mountable to form lanes of different widths. Each lane includes a primary rail on one side and a secondary rail on the other side for supporting and guiding at least one item (60) in the lane, and the primary rails each comprise at least one sensor (700) for sensing the item (60) in the lane.

Abstract: A storage system comprising: a cartridge, said cartridge including an upright having mounted to one side a plurality of guide blocks; a plurality of first drawer information means for providing information on at least one of position and movement of respective drawers; and a plurality of latch mechanisms, said system further comprising a plurality of drawers, each said drawer including: guide block engaging means extending in a depth direction of the drawer and mounting a first side of the drawer adjacent a respective guide block; second drawer information means disposed adjacent the first drawer information means; and at least one stop on the first side of the drawer for engaging with a said latch mechanism, wherein the latch mechanisms are electronically controllable, whereby access to the drawers can be controlled.

Abstract: A storage system comprising: a cartridge, said cartridge including an upright having mounted to one side a plurality of guide blocks; a plurality of first drawer information means for providing information on at least one of position and movement of respective drawers; and a plurality of latch mechanisms, said system further comprising a plurality of drawers, each said drawer including: guide block engaging means extending in a depth direction of the drawer and mounting a first side of the drawer adjacent a respective guide block; second drawer information means disposed adjacent the first drawer information means; and at least one stop on the first side of the drawer for engaging with a said latch mechanism, wherein the latch mechanisms are electronically controllable, whereby access to the drawers can be controlled.

Abstract: A storage system comprising: a cartridge, said cartridge including an upright having mounted to one side a plurality of guide blocks; a plurality of first drawer information means for providing information on at least one of position and movement of respective drawers; and a plurality of latch mechanisms, said system further comprising a plurality of drawers, each said drawer including: guide block engaging means extending in a depth direction of the drawer and mounting a first side of the drawer adjacent a respective guide block; second drawer information means disposed adjacent the first drawer information means; and at least one stop on the first side of the drawer for engaging with a said latch mechanism, wherein the latch mechanisms are electronically controllable, whereby access to the drawers can be controlled.

Abstract: A storage system comprising: a cartridge, said cartridge including an upright having mounted to one side a plurality of guide blocks; a plurality of first drawer information means for providing information on at least one of position and movement of respective drawers; and a plurality of latch mechanisms, said system further comprising a plurality of drawers, each said drawer including: guide block engaging means extending in a depth direction of the drawer and mounting a first side of the drawer adjacent a respective guide block; second drawer information means disposed adjacent the first drawer information means; and at least one stop on the first side of the drawer for engaging with a said latch mechanism, wherein the latch mechanisms are electronically controllable, whereby access to the drawers can be controlled.