Patents by Inventor Ian A. Harding

Ian A. Harding has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9714444
    Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: July 25, 2017
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
  • Patent number: 9492820
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Grant
    Filed: April 2, 2015
    Date of Patent: November 15, 2016
    Assignee: Applied Biosystems, LLC
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20160325284
    Abstract: In a high-density sequence detection system, an apparatus for transporting a microplate. A control system provides a thermocycler control signal to regulate a desired thermal output of a thermocycler block. A frame has a first side, a second side, and an opening that extends through the first side and the second side. A window is positioned in the opening and a circumferential seal is positioned around a periphery of the opening at the second side. The seal has a peripheral lip that seals against the microplate. A clamp is adapted to engage and displace the frame from an unclamped position to a clamped position, wherein in the unclamped position, the microplate is displaced away from the thermocycler block and in the clamped position, the clamp urges the microplate against the thermocycler block. In some embodiments the peripheral lip is positioned radially inward of the periphery of the opening.
    Type: Application
    Filed: January 8, 2016
    Publication date: November 10, 2016
    Inventors: Albert L. Camillo, Adrian Fawcett, Mike Y. Lu, Ian A. Harding
  • Patent number: 9052298
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Grant
    Filed: March 16, 2011
    Date of Patent: June 9, 2015
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20140162264
    Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
    Type: Application
    Filed: December 2, 2013
    Publication date: June 12, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
  • Patent number: 8597590
    Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
    Type: Grant
    Filed: May 27, 2010
    Date of Patent: December 3, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
  • Patent number: 8089623
    Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
    Type: Grant
    Filed: August 18, 2009
    Date of Patent: January 3, 2012
    Assignee: Applied Biosystems LLC
    Inventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
  • Publication number: 20110220239
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Application
    Filed: March 16, 2011
    Publication date: September 15, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. REED, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20110020179
    Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
    Type: Application
    Filed: May 27, 2010
    Publication date: January 27, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Min Yue, David M. Liu, Yuh-Min Chiang, Joon-Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Joy Roy, Maryam Shariati, Umberto Ulmanella
  • Publication number: 20100220321
    Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
    Type: Application
    Filed: August 18, 2009
    Publication date: September 2, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
  • Publication number: 20100221149
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries. Each of the plurality of capillaries can comprises an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.
    Type: Application
    Filed: November 25, 2009
    Publication date: September 2, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20100196212
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Application
    Filed: November 30, 2009
    Publication date: August 5, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20100113285
    Abstract: A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.
    Type: Application
    Filed: April 28, 2009
    Publication date: May 6, 2010
    Applicant: Life Technologies Corporation
    Inventors: H. Pin Kao, Ian A. Harding, Kathleen C. Hayashibara, Achim Karger, Fiona C. Laird Hyland, Mark F. Oldham, Omead Ostadan, Mark R. Pratt, Junko F. Stevens, Austin B. Tomaney, Greg Young
  • Patent number: 7695688
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Grant
    Filed: March 22, 2005
    Date of Patent: April 13, 2010
    Assignee: Applied Biosystems, LLC
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20100086977
    Abstract: In a high-density sequence detection system, an apparatus for transporting a microplate. A control system provides a thermocycler control signal to regulate a desired thermal output of a thermocycler block. A frame has a first side, a second side, and an opening that extends through the first side and the second side. A window is positioned in the opening and a circumferential seal is positioned around a periphery of the opening at the second side. The seal has a peripheral lip that seals against the microplate. A clamp is adapted to engage and displace the frame from an unclamped position to a clamped position, wherein in the unclamped position, the microplate is displaced away from the thermocycler block and in the clamped position, the clamp urges the microplate against the thermocycler block. In some embodiments the peripheral lip is positioned radially inward of the periphery of the opening.
    Type: Application
    Filed: September 3, 2009
    Publication date: April 8, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Albert L. Carrillo, Adrian Fawcett, Ian A. Harding, Mike Y. Lu
  • Patent number: 7586600
    Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: September 8, 2009
    Assignee: Applied Biosystem, LLC
    Inventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
  • Publication number: 20090214811
    Abstract: A sealing cover roll for use in sealing a microplate comprising an elongated carrier liner having a first surface and a second surface. A plurality of sealing covers are releasably carried on the first surface of the elongated carrier liner. Each of the plurality of sealing covers comprises a base stock and an adhesive. The adhesive is operable to retain the base stock to the elongated carrier liner and permit release of the base stock and the adhesive from the elongated carrier liner. A roll core supports a rolled configuration of the elongated carrier liner such that the first surface of the elongated carrier liner faces toward the roll core when rolled.
    Type: Application
    Filed: March 18, 2009
    Publication date: August 27, 2009
    Applicant: Life Technologies Corporation
    Inventors: Donald Sandell, Albert L. Carrillo, Ian A. Harding, Gary Lim
  • Publication number: 20090197277
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. A intermediate layer can be disposed between the assay input layer and the output layer. The intermediate layer can be flexible and comprise a plurality of microfluidic channels and a flow aperture being in fluid communication with the assay input port and the plurality of microfluidic channels.
    Type: Application
    Filed: April 2, 2009
    Publication date: August 6, 2009
    Applicant: Life Technologies Corporation
    Inventors: Nigel P. Beard, Albert L. Carrillo, Ian A. Harding, Mark T. Reed
  • Publication number: 20090176661
    Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.
    Type: Application
    Filed: March 19, 2009
    Publication date: July 9, 2009
    Applicant: APPLERA CORPORATION
    Inventors: Ian A. Harding, Donald Sandell, Kevin Bodner, Tim Woudenberg, Mark Oldham, H. Pin Kao, Gary Lim, John P. Bodeau, Adrian Fawcett, Robert C. Jones
  • Publication number: 20090081768
    Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.
    Type: Application
    Filed: September 21, 2007
    Publication date: March 26, 2009
    Applicant: Applera Corporation
    Inventors: Joon Mo Yang, David M. Liu, Yuh-Min Chiang, Carol Schrembri, Aldrich N.K. Lau, Umberto Ulmanella, Nigel P. Beard, Maryam Shariati, James C. Nurse, Douwe D. Haga, Ian A. Harding, Julio P. Focaracci