Abstract: Use of a non-ionic deep eutectic mixture consisting of A and B, A being R1R2N—CO—NR3R4 and B being selected from the group consisting of R5R6N—CO—CH3 and R7R8N—CO—NR9R10, and wherein each of R1-R10 is independently H, CH3 or alkyl, as a solvent or dispersant in chemical synthesis, material synthesis or fabrication, chemical or enzymatic catalysis, food, cosmetic or pharmaceutical formulation, separation or partitioning, heat transfer, and as detergents or cleaners, as well as such mixtures, is disclosed.

Abstract: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.

Abstract: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.

Abstract: The present invention relates to a method tor enriching and/or separating and/or immobilizing an analyte of interest comprising bringing an analyte of interest into contact with a derivatizing agent; incubating said analyte with said derivatizing agent, thereby incorporating a sulphonic acid group or as analogue thereof into the molecular structure of mo analyte of interest; bringing the analyte of interest into contact with a molecularly imprinted polymer with selective affinity foe a sulphonic acid group or an analogue thereof; and enriching and/or separating and/or immobilizing the analyte of interest by nee of the molecularly imprinted polymer. Further disclosed is a kit comprising a derivatizing agent, which contains a sulphonic group or an analogue thereof and a reactive group for creating a covalent bond between said derivatizing agent nod an analyte of interest, and a molecularly imprinted polymer with selective affinity for a sulphonic acid group or an analogue thereof.

Abstract: A polymer (e.g. a peptide) is produced by polymerizing monomers in the presence of a template, particularly a biologically active molecule or sample. The polymer is extracted and a soluble (preferably water-soluble) fraction is derived which specifically binds the template. Thus it can have complementary activity (e.g. biological). Applications include therapy, detection systems and separation systems.