Abstract: The present invention provides compositions and methods related to the production of fatty acids, such as triglycerides with genetically engineered cells, such as algae.

Abstract: Detector fusion molecules are produced by attaching a protein sub-unit to a linker, and attaching the linker to a nucleic acid molecule. The detector fusion molecules have utility in detecting and quantifying a specific target analyte from a sample. The protein sub-unit of the detector fusion molecule is selected to specifically bind the specific target analyte. The nucleic acid molecule of the detector fusion molecule is used as a tag, thus allowing for the detection and quantification of the target analyte. The sample is contacted with detector fusion molecules, thereby allowing detector fusion molecules to specifically bind any specific target analytes in the sample. The nucleic acid molecule of the detector fusion molecule is amplified using known processes, thereby producing an amplification product. The amplification product is detected and quantified, thus determining an amount of the target analyte in the sample.

Abstract: The present invention provides methods of covalently attaching a nucleic acid to a specific site on a binding polypeptide non-enzymatically. The methods produce large quantities of site specific nucleic acid coupled binding polypeptides. The site specific nucleic acid coupled binding polypeptides are used in accurate and sensitive methods for detecting and quantifying target analytes. Thus, the present invention provides economical and facile methods of making site specific nucleic acid coupled binding polypeptides for use in detection of target analytes.