Abstract: Disclosed are compositions for targeting 0TUD7A, the compositions having a component sufficient to block and/or reduce OTUD7A-mediated deubiquitination of EWS-FLI1 in a cell. The component can be 7Ai and variants thereof. The compositions can be used to treat Ewing sarcoma (EWS). Methods of using the disclosed compositions are also disclosed, including methods of treating EWS in a subject.

Abstract: Methods of extracting chromatin from tissue, such as formalin fixed, paraffin embedded (FFPE) tissue, are provided. The methods are rapid, simple, and preserve the chromatin signature. The methods can include, for example, removal of the tissue from the paraffin, enzymatic digestion of the extracellular matrix, and exposure to ultrasound energy, optionally in the presence of a cavitation enhancement agent, such as microbubbles, nanobubbles, and/or phase-change nanodroplets. The methods can also include a mechanical tissue dissociation step. The methods provide chromatin fragments that are free of enzyme bias from fragmentation by enzymes such as micrococcal nuclease (MNase) and that are of optimal size for further quantification and/or identification. Kits for extracting chromatin from tissue are also provided.

Abstract: A set of injection mold sections are supported and secured to parallel spaced platens of an injection molding press by quick-change locking couplers including a plurality of projecting knobs or studs on each mold section. The studs are received within corresponding cylinders each having a tapered seat which engages a mating surface on the stud. Each cylinder also supports circumferentially spaced balls movable between locked positions engaging enlarged head portions of the studs and retracted released positions in response to axial movement of a spring biased piston within a housing. The pistons are moved to released positions in response to pressurized air supplied simultaneously to the housings. The locking couplers may be recessed within an adapter plate disposed between a mold section and the supporting platen, and the adapter plate has a pattern of threaded holes for mounting a conventional mold section.