Patents by Inventor Ian M. White
Ian M. White has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230063411Abstract: Disclosed are liquid and solid assay compositions and portable sample reader devices for use in a sample-to-answer diagnostic system for the detection of one or more analytes, preferably the detection of circulating histones in whole blood. Further provided are methods of making and using the assay compositions and portable sample reader, including the collection of a raw sample, testing the sample using the assay compositions, and analyzing the sample via the portable sample reader. More particularly, assay compositions comprising a sacrificial partition, target molecule, detectable label, and sacrificial partition are used in combination with a sample reader comprising an optical system and a housing unit as part of a sample-to-answer diagnostic system for quantifying circulating histones in whole blood as a mechanism of predicting the risk of multiple organ failure.Type: ApplicationFiled: July 8, 2022Publication date: March 2, 2023Inventors: Micaela Everitt, David Boegner, Ian M. White
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Publication number: 20220290208Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow pathogen cell lysis. In one embodiment, the microfluidic device comprises a microfluidic channel, a microporous structure within the channel, and an enzyme immobilized on the surface of the microporous structure configured to lyse pathogen cells in fluid flowing through the microfluidic channel.Type: ApplicationFiled: May 27, 2022Publication date: September 15, 2022Applicants: Canon U.S.A., Inc., UNIVERSITY OF MARYLANDInventors: Ian M. White, Stephen Restaino, Marina Pranda
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Publication number: 20220213465Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.Type: ApplicationFiled: November 15, 2021Publication date: July 7, 2022Applicants: University of Maryland, College Park, Canon U.S.A., Inc.Inventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit, Imaly Nanayakkara, Weidong Cao
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Patent number: 11345944Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow pathogen cell lysis. In one embodiment, the microfluidic device comprises a microfluidic channel, a microporous structure within the channel, and an enzyme immobilized on the surface of the microporous structure configured to lyse pathogen cells in fluid flowing through the microfluidic channel.Type: GrantFiled: December 30, 2014Date of Patent: May 31, 2022Assignees: Canon U.S.A., Inc., University of MarylandInventors: Ian M. White, Stephen Restaino, Marina Pranda
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Patent number: 11174478Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.Type: GrantFiled: May 20, 2016Date of Patent: November 16, 2021Assignees: University of Maryland, College Park, Canon U.S.A., Inc.Inventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit, Imaly Nanayakkara, Weidong Cao
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Patent number: 10823648Abstract: A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.Type: GrantFiled: October 30, 2017Date of Patent: November 3, 2020Assignee: University of MarylandInventors: Jeffrey M Burke, Ian M White
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Patent number: 10675624Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.Type: GrantFiled: January 18, 2019Date of Patent: June 9, 2020Assignee: University of Maryland, College ParkInventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
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Patent number: 10443086Abstract: The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“PCR”) mixtures. The class of surfactants consists of those having the chemical formula R—(OCH2CH2)n—OH, wherein R is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25. The present invention also relates to methods, devices, systems, and kits incorporating the above-described class of surfactants.Type: GrantFiled: December 30, 2014Date of Patent: October 15, 2019Assignee: University of Maryland, College ParkInventors: Ian M. White, Kunal Pandit, Srinivasa Raghavan
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Patent number: 10393753Abstract: A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.Type: GrantFiled: March 10, 2017Date of Patent: August 27, 2019Assignee: University of Maryland, College ParkInventors: Donald K. Milton, Ian M. White
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Publication number: 20190224672Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.Type: ApplicationFiled: January 18, 2019Publication date: July 25, 2019Applicant: University of Maryland, College ParkInventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
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Publication number: 20190091984Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.Type: ApplicationFiled: November 26, 2018Publication date: March 28, 2019Applicant: University of Maryland, College ParkInventors: Ian M. White, Jeffrey Burke, Kunal Pandit
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Patent number: 10183291Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.Type: GrantFiled: December 29, 2017Date of Patent: January 22, 2019Assignee: University of Maryland, College ParkInventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
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Patent number: 10137673Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.Type: GrantFiled: December 30, 2014Date of Patent: November 27, 2018Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ian M. White, Jeffrey Burke, Kunal Pandit
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Publication number: 20180117589Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.Type: ApplicationFiled: December 29, 2017Publication date: May 3, 2018Applicant: University of Maryland, College ParkInventors: Ian M. White, Srinivasa Raghavan, KunaI R. Pandit
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Publication number: 20180045626Abstract: A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.Type: ApplicationFiled: October 30, 2017Publication date: February 15, 2018Applicant: University of MarylandInventors: Jeffrey M. Burke, Ian M. White
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Patent number: 9855555Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.Type: GrantFiled: May 20, 2016Date of Patent: January 2, 2018Assignee: University of MarylandInventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
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Patent number: 9804068Abstract: A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.Type: GrantFiled: September 30, 2013Date of Patent: October 31, 2017Assignee: University of MarylandInventors: Jeffrey M Burke, Ian M White
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Publication number: 20170184609Abstract: A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.Type: ApplicationFiled: March 10, 2017Publication date: June 29, 2017Applicant: University of Maryland, College ParkInventors: Donald K. Milton, Ian M. White
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Patent number: 9617582Abstract: A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.Type: GrantFiled: September 4, 2013Date of Patent: April 11, 2017Assignee: University of Maryland College ParkInventors: Donald K. Milton, Ian M. White
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Publication number: 20160340668Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.Type: ApplicationFiled: May 20, 2016Publication date: November 24, 2016Applicants: UNIVERSITY OF MARYLAND, CANON U.S. LIFE SCIENCES, INC.Inventors: IAN M. WHITE, SRINIVASA RAGHAVAN, KUNAL R. PANDIT, IMALY NANAYAKKARA, WEIDONG CAO