Abstract: This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH3)6]3+, or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry.

Abstract: This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH3)6]3+, or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry.

Abstract: In Bilaterally Generated Variable Instant Password system, Variable character sets or Master Variable Character Set with Sub Variable Character Sets of any level containing Character Units are used as means of generating Passwords. Password is a random combination of Character Units of Variable Character Set/derivatives, which is generated by a call of random numbers from SERVICE PROVIDER and corresponding response of USER. Bilaterally Generated Variable Instant Passwords and Non Repeating Bilaterally Generated Variable Instant Passwords are the two types of passwords that can be generated in this system. Font properties differentiation provides high password variability. Transformation of Variable Character Sets is also used to safeguard passwords. This system can authenticate persons, objects, individual actions initiated by USERs through separate passwords.