Abstract: Provided is a cell image analysis method including: a morphological information image acquisition step of acquiring a morphological information image including morphological information of a cell; an autofluorescence image acquisition step of acquiring an autofluorescence image which is obtained by photographing autofluorescence of the cell and which includes the same field of view as in the morphological information image; a cell region extraction step of extracting one or more cell regions from the morphological information image; and a luminance information extraction step of extracting pieces of luminance information of two or more wavebands different from one another from the autofluorescence image, with respect to the one or more cell regions.

Abstract: An apparatus acquiring an image of a subject of a phase object includes: a first light irradiation unit irradiating the subject with a parallel light as a first light; a second light irradiation unit irradiating the subject with a second light at an incidence angle different from that of the first light irradiation unit; an imaging lens having a telecentric optical system; and an imaging unit which images the subject irradiated with at least one of the first light and the second light through the imaging lens, wherein the optical axis of the imaging lens is parallel to an optical path of the first light irradiation unit, the imaging unit is arranged on the optical axis opposite to the first light irradiation unit to the subject, and an optical path length adjusting unit controlling the distance between the telecentric optical system and the subject on the optical axis is arranged.

Abstract: An image acquisition system is configured to acquire an image of a floating cell included in a cell suspension within a vessel, and includes a transmitted light image capturing unit configured to acquire a transmitted light image of the floating cell, a fluorescence image capturing unit configured to acquire a fluorescence image of the floating cell, and an information acquisition unit configured to acquire information about an image capturing condition for the fluorescence image using a first transmitted light image captured at a first time and a second transmitted light image captured at a second time different from the first time.

Abstract: A container inspection method for inspecting a container includes a detection step of sequentially detecting an AE wave generated in a container by an AE sensor while increasing the pressure inside the container, a calculation step of sequentially calculating dimensionless AE average energy by dividing AE average energy by predetermined AE specific average energy, and a determination step of determining whether an AE wave having dimensionless AE average energy exceeding a predetermined threshold indicating a sign of breakage of the container is observed until the pressure inside the container reaches a predetermined inspection pressure upper limit.

Abstract: A cell image processing system includes an image acquisition unit configured to acquire a cell image including a plurality of cells as a subject, a cell region extraction unit configured to extract a plurality of cell regions from the cell image, a cell information acquisition unit configured to acquire characteristic information including morphological information about each of the cell regions and pixel information about each of the cell regions, a representative cell determination unit configured to determine a representative cell region from among the plurality of extracted cell regions based on at least one type of information constituting the characteristic information, and a display control unit configured to cause a display unit to display image information about a cell region corresponding to the representative cell, positional information corresponding to the representative cell region, and characteristic information about a cell region corresponding to the representative cell.

Abstract: A cell observation system has: a part for a culturing container to be placed, the culturing container having a surface where cells are culturable and an optically transparent side face forming a peripheral wall of the surface; a light emission unit configured to irradiate the surface with irradiation light from the side face; and an image capturing unit configured to capture images of the surface and has at least any of features (a) controlling variation in an amount of light of the irradiation light, the surface being irradiated with the irradiation light, and the variation being due to absorption of light by a medium in the culturing container and (b) correcting an effect caused by variation in an amount of light of the irradiation light, the surface being irradiated with the irradiation light, and the variation being due to absorption of light by a medium in the culturing container.

Abstract: An imaging apparatus for phase objects includes an imaging unit that images a phase object through an imaging lens, a light irradiation unit including a collimating unit that converts a light ray emitted from a light source into a substantially parallel light ray and a branching prism that branches and outputs the substantially parallel light ray such that the phase object is irradiated with the branched substantially parallel light rays in a plurality of different directions that are not parallel to an optical axis of the imaging lens, and a placement unit on which the phase object is placed in a region in which the light rays output in the plurality of different directions by the light irradiation unit first intersect each other.

Abstract: A cell culture system includes: a cell culture apparatus including: a placement table placing a culture vessel having a culture surface on which cell culture is viable and a side surface forming a peripheral wall around the culture surface, the side surface being optically transparent; a supply pipe allowing liquid to be supplied into the culture vessel; and a discharge pipe allowing liquid to be discharged from the culture vessel; an image pickup portion allowing observation of the culture surface; and a light irradiation portion emitting illumination light toward the side surface, wherein the supply pipe and the discharge pipe are arranged so that an openings thereof are located in proximity to points on an inner side surface of the culture vessel, which define a width of the culture surface in a direction orthogonal to an optical axis of the illumination light.

Abstract: Provided is a cell detachment system including: a scraper having magnetism, for detaching cells adhering to a culture surface; a magnetic field applying unit configured to apply a magnetic field to the scraper; and a scraper position control unit configured to control a position of the scraper by controlling the magnetic field applying unit, wherein the scraper is configured to move while rotating under a state in which no magnetic field is applied thereto, and under a state in which a magnetic field is applied thereto by the magnetic field applying unit, to be caused to move, without rotating, by the scraper position control unit so as to detach the adhering cells.

Abstract: Provided are a cell culture system and a cell culture method that facilitate estimation of the number of cells after culture. The cell culture system includes: a culture substrate in which a plurality of depressions are arranged and to which cells are caused to adhere; a scraper configured to detach cells adhering to a region other than the depressions on the culture substrate by sliding on the culture substrate and being brought into contact with the region other than the depressions on the culture substrate; and a cell removal mechanism configured to remove, from the culture substrate, the cells detached by the scraper.

Abstract: A cell information acquisition method of acquiring information on a cell by using autofluorescence emitted from the cell includes a metabolic status changing step of, by changing a metabolic status in the cell, changing at least one of a first autofluorescence luminance indicating a metabolic status of a first metabolic pathway and a second autofluorescence luminance indicating a metabolic status of a second metabolic pathway different from the first metabolic pathway, and an information acquisition step of, after the metabolic status changing step, acquiring information on the cell by using information on the first autofluorescence luminance and information on the second autofluorescence luminance.

Abstract: Provided is a method of stably reprogramming a cell. Provided is an information processing method for reprogramming of a blood cell, the information processing method including an autofluorescence information acquisition step of acquiring autofluorescence information on a subject blood cell and an analysis step of performing analysis based on the autofluorescence information and cell information on the subject blood cell, in which the cell information includes identification information on the blood cell.

Abstract: An apparatus acquiring an image of a subject of a phase object includes: a first light irradiation unit irradiating the subject with a parallel light as a first light; a second light irradiation unit irradiating the subject with a second light at an incidence angle different from that of the first light irradiation unit; an imaging lens having a telecentric optical system; and an imaging unit which images the subject irradiated with at least one of the first light and the second light through the imaging lens, wherein the optical axis of the imaging lens is parallel to an optical path of the first light irradiation unit, the imaging unit is arranged on the optical axis opposite to the first light irradiation unit to the subject, and an optical path length adjusting unit controlling the distance between the telecentric optical system and the subject on the optical axis is arranged.

Abstract: Provided is a method that addresses a problem of classifying cells through use of cell autofluorescence, the method including the steps of: acquiring autofluorescence information regarding a plurality of cells through use of an autofluorescence image regarding the plurality of cells; acquiring region information; extracting the autofluorescence information on individual cells from the autofluorescence information regarding the plurality of cells and the region information; and classifying the plurality of cells into two or more groups by performing clustering through use of the autofluorescence information on the individual cells.

Abstract: An apparatus acquiring an image of a subject of a phase object includes: a first light irradiation unit irradiating the subject with a parallel light as a first light; a second light irradiation unit irradiating the subject with a second light at an incidence angle different from that of the first light irradiation unit; an imaging lens having a telecentric optical system; and an imaging unit which images the subject irradiated with at least one of the first light and the second light through the imaging lens, wherein the optical axis of the imaging lens is parallel to an optical path of the first light irradiation unit, the imaging unit is arranged on the optical axis opposite to the first light irradiation unit to the subject, and an optical path length adjusting unit controlling the distance between the telecentric optical system and the subject on the optical axis is arranged.

Abstract: According to one embodiment, a culture vessel includes a rigid bottom part and a wall part. The wall part has a culture medium inlet and includes a deformable portion. The culture vessel allows the deformable portion to deform so as to change the capacity of the culture vessel when a pressure of the internal space of the culture vessel varies.

Abstract: There are suitability and unsuitability of a shape of a substrate used for cell culture, for each step in various cell culture protocols, and it has been difficult to continue providing a culture substrate shape suitable for each step. Provided is a cell culture system including: a culture sheet deformable by external force, to which a cell can adhere and be cultured; a die member configured to be pressed against the culture sheet to deform a shape of the culture sheet into a randomly-selected shape; an optical device configured to observe a state of the cell adhering onto the culture sheet; and a drive unit configured to move the die member, to change a randomly-selected portion of the culture sheet into a shape along the die member or release the culture sheet from deformation.

Abstract: A seal space is formed between first and second metal separators of a power generation cell. A device body is disposed in the seal space. The device body includes a voltage measurement sensor and a transmission mechanism. The voltage measurement sensor measures the voltage generated during operation of the power generation cell. The transmission mechanism transmits information of the voltage measured by the voltage measurement sensor via wireless communication. The voltage measurement sensor and the transmission mechanism are embedded in an electromagnetic/heat resistant shield member, and attached to a surface of the second metal separator.

Abstract: A seal space is formed between first and second metal separators of a power generation cell. A device body is disposed in the seal space. The device body includes a voltage measurement sensor and a transmission mechanism. The voltage measurement sensor measures the voltage generated during operation of the power generation cell. The transmission mechanism transmits information of the voltage measured by the voltage measurement sensor via wireless communication. The voltage measurement sensor and the transmission mechanism are embedded in an electromagnetic/heat resistant shield member, and attached to a surface of the second metal separator.

Abstract: A given visual field is divided into a plurality of areas, and brightness information in the areas is detected at predetermined time intervals and stored as information of a mobile body in a memory of a motor vehicle monitoring apparatus on a motor vehicle. The bright information detected in the areas at different times is then read from the memory, and a motion of the brightness information between adjacent ones of the areas is detected as a one-dimensional optical flow. The detected one-dimensional optical flow is compared with a one-dimensional optical flow generated from a road due to the speed of the motor vehicle, for thereby detecting another motor vehicle which is moving toward the motor vehicle.