Abstract: A solution sample holding method between two light-transmitting plate-like members for measuring light passing through a minute amount of solution sample, comprises a dripping step and a covering step. The dripping step is to drip a minute amount of solution sample in a drip area of a sample mounting face of a first light-transmitting member. The sample mounting face also includes a liquid-repellent area surrounding the drip area. The covering step is to cover the solution sample with a second light-transmitting member and to maintain a predetermined distance between the first light-transmitting member and the second light-transmitting member. The liquid-repellent area of the sample mounting face is covered with a liquid-repellent substance. The minute amount of solution sample is held in contact with the two light-transmitting members.