Abstract: The present disclosure relates to conferring desirable agronomic traits in Legume plants. More particularly, the current invention pertains to producing Legume plants with improved traits by manipulating genes controlling plant architecture.

Abstract: The present invention discloses a modified Soybean plant, plant part, or plant cell exhibiting reduced phytoestrogen content and/or increased protein content, wherein the modified Soybean plant, plant part, or plant cell comprises one or more gene editing derived mutations in a least one gene involved in phytoestrogen biosynthesis and/or protein accumulation. Methods for producing the same are further disclosed.

Abstract: Provided is a Cannabis plant exhibiting altered tetrahydrocannabinolic acid (THCA) and/or cannabidiolic acid (CBDA) content. The plant includes a modified genomic locus involved in tetrahydrocannabinolic acid synthase (THCAS) and/or cannabidiolic acid synthase (CBDAS) gene expression, the genomic locus includes at least one targeted nucleotide modification within a regulatory region modulating the expression of at least one allele of the THCAS and/or CBDAS genes. Further provided are methods for producing the aforementioned Cannabis plant.

Abstract: Provided relates to conferring desirable agronomic traits in Cucumber plants. Further, provided is a modified Cucumber plant exhibiting at least one improved domestication trait. The modified Cucumber plant includes at least one genetic modification conferring reduced expression of at least one Cucumber SELF PRUNING (SP) (CuSP) gene. Also, further provided are methods for producing the aforementioned modified Cucumber plant and uses thereof.

Abstract: The present invention discloses a modified Cannabis plant exhibiting enhanced resistance to powdery mildew (PM). The aforementioned modified Cannabis plant comprises a mutated Cannabis mlo1 (Csmlo1) allele comprising a genomic modification selected from an indel of 14 bp at position corresponding to position 12 of SEQ ID NO: 882, or a fraction thereof, or a nucleic acid insertion at position corresponding to position 104-105 of SEQ ID NO: 882, or a combination thereof. The present invention further discloses methods for production of the modified Cannabis plant using genome modification.

Abstract: Provided is a modified Cannabis plant exhibiting reduced volatile organic compounds (VOCs) emission. The modified Cannabis plant includes at least one targeted gene modification conferring reduced expression or silencing of at least one gene involved in a terpene biosynthesis pathway. Further provided are methods and uses concerning the aforementioned modified Cannabis plant.

Abstract: The present invention discloses a modified Cannabis plant exhibiting at least one improved domestication trait compared with wild type Cannabis, wherein the modified plant comprises at least one mutated Cannabis SELF PRUNING (SP) (CsSP) gene and/or at least one mutated Cannabis SELF PRUNING 5G (SP5G) (CsSP5G) gene. The present invention further discloses methods for production of the aforementioned modified Cannabis plant using genome modification.

Abstract: The present invention discloses a method for increasing flower yield in Cannabis plants via genome editing approach. More particularly, the method comprises steps of: (a) selecting a gene involved in the flowering pathways of said Cannabis species; (b) synthesizing or designing a gRNA expression cassette corresponding to a targeted cleavage locus along the Cannabis genome; (c) transforming said Cannabis plant cells to insert genetic material into them; (d) culturing said Cannabis plant cells; (e) selecting said Cannabis cells which express desired mutations in the editing target region, and (f) regenerating a plant from said transformed plant cell, plant cell nucleus, or plant tissue.

Abstract: A modified Cannabis plant exhibiting enhanced resistance to powdery mildew (PM). The aforementioned modified Cannabis plant comprises a targeted genome modification conferring reduced expression of at least one Cannabis MLO (CsMLO) allele as compared to a Cannabis plant lacking said targeted genome modification. Methods for production of the modified Cannabis plant using genome modification.

Abstract: Provided is a Cannabis plant with reduced delta-9-tetrahydrocannabinol (THC) content, or reduced cannabidiol (CBD) content, or reduced THC and CBD content. According to a core aspect of the invention, the Cannabis plant comprises at least one targeted genome modification effective in decreasing expression of. at least one Cannabis gene encoding a cannabinoid biosynthesis enzyme selected from the group consisting of Cannabis tetrahydrocannabinolic acid synthase (CsTHCAS), Cannabis cannabidiolic acid synthase (CsCBDAS), Cannabis aromatic prenyltransferase (CsPT), Cannabis olivetol synthase (CsOLS), Cannabis acyl-activating enzyme 1 (CsAAE1) and any combination thereof. Further disclose are methods for production of the Cannabis plants and use thereof.

Abstract: Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

Abstract: Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

Abstract: Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.