Patents by Inventor Igor A. Kozlov

Igor A. Kozlov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA
    Patent number: 11739382
    Abstract: Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, competitive blocking oligonucleotides, and probes targeting HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA).
    Type: Grant
    Filed: February 24, 2020
    Date of Patent: August 29, 2023
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Jeffery Fong, Aaron T. Hamilton, Marintha Heil, Igor Kozlov, Ed Gustavo Marins, Elizabeth Marie Scott, Ling Wang
  • DETECTION OF TARGET NUCLEIC ACID BY SOLID-PHASE MOLOGRAPHY
    Publication number: 20220298555
    Abstract: The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography.
    Type: Application
    Filed: December 19, 2019
    Publication date: September 22, 2022
    Inventors: Dieter Heindl, Igor Kozlov, Nikolaus-Peter Stengele, Alison Tsan, Volker Gatterdam
  • COMPOSITIONS AND METHODS FOR AMPLIFICATION AND DETECTION OF HEPATITIS B VIRUS RNA, INCLUDING HBV RNA TRANSCRIBED FROM cccDNA
    Publication number: 20220275465
    Abstract: Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, competitive blocking oligonucleotides, and probes targeting HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA).
    Type: Application
    Filed: January 28, 2022
    Publication date: September 1, 2022
    Inventors: Alan Blair, Jeffery Fong, Aaron T. Hamilton, Marintha Heil, Igor Kozlov, Ed Gustavo Marins, Elizabeth Marie Scott, Ling Wang
  • PEPTIDE CONSTRUCTS AND ASSAY SYSTEMS
    Publication number: 20220260581
    Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
    Type: Application
    Filed: April 22, 2022
    Publication date: August 18, 2022
    Applicant: Prognosys Biosciences, Inc.
    Inventors: Igor A. KOZLOV, Mark S. CHEE, Petr CAPEK, David A. ROUTENBERG
  • Methods for performing multiplexed real-time PCR
    Patent number: 11345958
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: May 7, 2021
    Date of Patent: May 31, 2022
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Peptide constructs and assay systems
    Patent number: 11340232
    Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: May 24, 2022
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, Mark S. Chee, Petr Capek, David A. Routenberg
  • Methods for performing multiplexed real-time PCR
    Patent number: 11198903
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: October 7, 2019
    Date of Patent: December 14, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20210269857
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: May 7, 2021
    Publication date: September 2, 2021
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Photocleavable nucleotide reagents with high stability
    Patent number: 11078534
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: August 3, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • Methods for performing multiplexed real-time PCR
    Patent number: 11034997
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 15, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed PCR
    Patent number: 11028433
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 8, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • COMPOSITIONS AND METHODS FOR AMPLIFICATION AND DETECTION OF HEPATITIS B VIRUS RNA, INCLUDING HBV RNA TRANSCRIBED FROM cccDNA
    Publication number: 20210062259
    Abstract: Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, competitive blocking oligonucleotides, and probes targeting HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA).
    Type: Application
    Filed: February 24, 2020
    Publication date: March 4, 2021
    Inventors: Jeffery Fong, Aaron T. Hamilton, Marintha Heil, Igor Kozlov, Ed Gustavo Marins, Elizabeth Marie Scott, Ling Wang
  • Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch
    Patent number: 10590469
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: March 17, 2020
    Assignee: Roche Molecular Svstems, Inc.
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20200048682
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: October 7, 2019
    Publication date: February 13, 2020
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • PHOTOCLEAVABLE NUCLEOTIDE REAGENTS WITH HIGH STABILITY
    Publication number: 20190161797
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: November 26, 2018
    Publication date: May 30, 2019
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • Polynucleotide conjugates and methods for analyte detection
    Patent number: 10288608
    Abstract: The present disclosure provides polynucleotide conjugates, methods, and assay systems for use in detecting the presence, absence, and/or amount of an analyte in a sample. Various polynucleotide conjugates, conjugate pairs, sets, libraries, and assay systems comprising the same are disclosed. In particular, methods and assay systems for antibody detection and analysis are provided. For example, assays capable of high levels of multiplexing are used for antibody detection and analysis in a biological sample, e.g., Lyme disease patient samples. The presently disclosed polynucleotide conjugates, methods, and assay systems can be used to provide sensitive and reliable diagnosis, even at early stages of a disease or condition. Use for monitoring disease progression and prognosis is also disclosed.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: May 14, 2019
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, John Andrew Altin, Petr Capek, Mark S. Chee
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR IN A SELF-CONTAINED NUCLEIC ACID ANALYSIS POUCH
    Publication number: 20190024155
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Application
    Filed: September 25, 2018
    Publication date: January 24, 2019
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • PEPTIDE CONSTRUCTS AND ASSAY SYSTEMS
    Publication number: 20180328936
    Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
    Type: Application
    Filed: April 30, 2018
    Publication date: November 15, 2018
    Applicant: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, Mark S. Chee, Petr Capek, David A. Routenberg
  • Peptide constructs and assay systems
    Patent number: 9958454
    Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
    Type: Grant
    Filed: October 31, 2013
    Date of Patent: May 1, 2018
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, Mark S. Chee, Petr Capek, David A. Routenberg
  • METHODS AND COMPOSITIONS FOR PURIFYING DOUBLE STRANDED NUCLEIC ACIDS
    Publication number: 20180073011
    Abstract: Provided herein are methods and compositions to effectively isolate short, double-stranded nucleic acids on magnetic glass particles.
    Type: Application
    Filed: September 12, 2017
    Publication date: March 15, 2018
    Inventors: Priya Bellare, Cherie Holcomb, Alan Casilla, Qiao Ruan, Trevor Hryce, Karan Kampani, Igor Kozlov