Abstract: This disclosure relates to the production of opium poppy plants having high levels of thebaine. More particularly, the disclosure relates to the production of opium poppies having high levels of thebaine by simultaneously reducing the expression of genes encoding thebaine 6-0-demethylase (T60DM) and codeine 3-0-demethylase (CODM).

Abstract: The present invention provides methods of extraction of at least one cannabinoid from an initial cannabis biomass in “one-pot” step using toluene to form a toluene extract and using the toluene extract for producing high concentrations of ?-9-tetrahydrocannabinol (?9THC) and/or cannabinol (CBN) of a purity of at least 75% and at a yield of at least 75% by weight of the at least one cannabinoid from the initial cannabis biomass in the toluene extract.

Abstract: This disclosure relates to the production of opium poppy plants having high levels of thebaine. More particularly, the disclosure relates to the production of opium poppies having high levels of thebaine by simultaneously reducing the expression of genes encoding thebaine 6-0-demethylase (T60DM) and codeine 3-0-demethylase (CODM).

Abstract: The present invention provides systems, methods and software for assessment of the personalized efficacy of cannabis drug for treatment of various diseases, disorders, syndromes and conditions, based on analysis of high-throughput gene expression profiling. According to signaling pathway topology, the gene expression profiles are convoluted into signaling pathway activities using an SPIA method. The drug action as evaluated by comparison between samples of the disease states before and after treatment with cannabis drug, as well as control samples and providing an individual with a treatment having a cannabis drug efficiency index (CDEI) greater than 0.

Abstract: The present invention provides methods of extraction of at least one cannabinoid from an initial cannabis biomass in “one-pot” step using toluene to form a toluene extract and using the toluene extract for producing high concentrations of ?-9-tetrahydrocannabinol (?9THC) and/or cannabinol (CBN) of a purity of at least 75% and at a yield of at least 75% by weight of the at least one cannabinoid from the initial cannabis biomass in the toluene extract.

Abstract: The present invention provides new unique Cannabis lines, extracts and methods for their use in anti-cancer therapies and modalities. The method includes generation of unique lines, whole plant extract preparation, treating cancer cells and normal cells with extracts in amounts sufficient to kill cancer cells while sparing normal ones. The modulation of cell proliferation, growth and death results in efficient elimination of cancer cells.

Abstract: The present invention provides new unique cannabis lines, extracts and methods for their use in anti-inflammatory therapies and modalities. The method includes generation of unique lines, whole plant extract preparation, treating normal human 3D tissues with UV to induce inflammation, and then with extracts in amount sufficient to profoundly down-regulate inflammation and molecular pathways involved in rheumatoid arthritis (RA), irritable bowel disease (IBD) and other auto-inflammatory disorders. The modulation of these pathways is a key to treatment success in RA, IBD and the other auto-inflammatory disorders.

Abstract: The present invention provides new Cannabis lines, extracts and methods for skin rejuvenation and healing and protection against harmful environmental factors such as UV rays and other damaging agents. The method includes generation of unique lines, whole plant extract preparation, exposing human 3D skin tissues to UV and treating human 3D skin tissues with extracts in amount sufficient to modulate gene expression in the skin tissues before and after UV exposure. The modulation of gene expression then results in a reduction of the disease state-associated changes or aspects thereof in the cannabis-treated skin tissues.

Abstract: The present invention provides cannabis lines, extracts and methods for the treatment of inflammatory skin disorders such as psoriasis, eczema, melanoma skin cancer, and others. The method includes generation of unique lines, whole plant extract preparation, treating human 3D skin tissues and disease models with extracts in amount sufficient to modulate gene expression in the skin tissues. The modulation of gene expression then results in a reduction of the disease state-associated changes or aspects thereof in the treated skin tissues.

Abstract: This disclosure relates to the production of opium poppy plants having high levels of thebaine. More particularly, the disclosure relates to the production of opium poppies having high levels of thebaine by simultaneously reducing the expression of genes encoding thebaine 6-0-demethylase (T60DM) and codeine 3-0-demethylase (CODM).

Abstract: A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells, and rye cells.

Abstract: A plant culture medium composition for modulating plant transformation events, comprising a plant culture medium and an effective amount of at least one compound having a rare earth element component intermixed thereinto. The at least one rare earth element-containing compound is selected from the group comprising: CeCl3, LaCl3, and combinations thereof. Another embodiment relates to a method for modulating the frequency of plant transformation events. The method comprises the steps of providing the plant culture medium composition and contacting at least one plant with the plant culture medium composition. At least one cell from the at least one plant is transformed with a nucleic acid of interest. The presence of at least one transformation event is detected and quantified. The frequency of quantified transformation events is compared with a suitable control. Changes in quantified transformations events compared to the control are indicative of changes in the frequency of plant transformation events.

Abstract: A plant culture medium composition for modulating plant transformation events, comprising a plant culture medium and an effective amount of at least one compound having a chloride component intermixed thereinto. In one embodiment, the at least one chloride-containing compound is selected from the group comprising: NaCl, MgCl2, and KCl. Another embodiment relates to a method for modulating the frequency of plant transformation events. The method comprises the steps of providing a plant culture medium composition and contacting at least one plant with the plant culture medium composition. At least one cell from the at least one plant is transformed with a nucleic acid of interest. The presence of at least one transformation event is detected and quantified. The frequency of quantified transformation events is compared with a suitable control. Changes in quantified transformations events compared to the control are indicative of changes in the frequency of plant transformation events.

Abstract: A composition, and method for enhancing transformation of plants is provided. The method involves the use of a transformation-enhancing composition enriched with an effective amount of ammonium nitrate such that, when contacted with plants, it produces an increase in homologous recombination and transformation frequency in the plants. The composition, method and use of ammonium nitrate as described herein reduces undesirable deletions of the introduced DNA.