Abstract: The invention relates to the field of medicine and genetic engineering, specifically to guide RNAs and DNA fragments and to cell selection methods, which can be used in CRISPR-Cas9 systems for producing lines of natural killer cells with a PD-1 knockout gene and increased production of TRAIL or Fas-ligand proteins. Methods are disclosed for producing modified lines of NK cells: with a PD-1 knockout gene and constitutive Fas-ligand overexpression, with a PD-1 knockout gene and constitutive TRAIL overexpression, and also a method is described for selecting modified NK cells with a PD-1 knockout gene, which is carried out using a zeocin selection marker. The invention makes it possible to produce a high yield of modified lines of NK cells that are highly active in inhibiting the growth of tumour cells.

Abstract: A multifunctional device for measuring fluorescence, luminescence and light transmission for diagnostics. A sample carrier is designed in the form of a biochip, cell, pan or microplate. The device comprises a first and second group of screens mounted behind the rear surface of a sample solid carrier. Light sources of the sample are provided with light-absorbing elements for suppressing light reflected from the front surface of the sample carrier and from screen surfaces. Screen holders allow for alternatively mounting light reflective/retroreflective screens to maximize fluorescent or luminescent signal. A diffusing screen measures light transmission through the sample. Light-absorbing screens behind the rear surface of the sample and light-absorbing elements on light sources from the sample's top surface, increase signal-to-noise ratio. Said device permits measuring signals on biochip surfaces and in solutions during hybridization or amplification reactions.