Patents by Inventor Ikuo Matsui
Ikuo Matsui has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8105814Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.Type: GrantFiled: August 25, 2010Date of Patent: January 31, 2012Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Ikuo Matsui, Yuji Urushibata
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Publication number: 20110014679Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.Type: ApplicationFiled: August 25, 2010Publication date: January 20, 2011Applicant: National Inst. of Adv Industrial Science and Tech.Inventors: Ikuo MATSUI, Yuji Urushibata
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Patent number: 7846704Abstract: A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.Type: GrantFiled: January 21, 2005Date of Patent: December 7, 2010Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Eriko Matsui, Ikuo Matsui
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Patent number: 7824886Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.Type: GrantFiled: May 15, 2007Date of Patent: November 2, 2010Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Ikuo Matsui, Yuji Urushibata
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Publication number: 20080118922Abstract: A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.Type: ApplicationFiled: January 21, 2005Publication date: May 22, 2008Applicant: National Institute of advanced industrial science and technologyInventors: Eriko Matsui, Ikuo Matsui
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Publication number: 20070264660Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.Type: ApplicationFiled: May 15, 2007Publication date: November 15, 2007Applicant: National Inst of Adv Industrial Science and Tech.Inventors: Ikuo Matsui, Yuji Urushibata
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Patent number: 6960454Abstract: The invention relates to a thermophilic enzyme having ?-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.Type: GrantFiled: August 6, 1999Date of Patent: November 1, 2005Assignee: Director-General of Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi
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Patent number: 6939699Abstract: The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.Type: GrantFiled: February 26, 2003Date of Patent: September 6, 2005Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Ikuo Matsui, Hiroki Higashibata, Yutaka Kawarabayashi
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Publication number: 20040161826Abstract: The invention is directed to novel thermostable aminotransferases useful in synthesizing an amino acid derivative with high optical purity, and nucleic acids encoding the enzyme. The invention also includes antibodies that specifically bind to the aminotransferases of the invention.Type: ApplicationFiled: March 26, 2004Publication date: August 19, 2004Applicant: National Institute of Advanced Industrial Science and Technology, a Tokyo, Japan corporationInventors: Ikuo Matsui, Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi, Eriko Matsui
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Publication number: 20030232357Abstract: The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.Type: ApplicationFiled: February 26, 2003Publication date: December 18, 2003Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Ikuo Matsui, Hiroki Higashibata, Yutaka Kawarabayashi
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Publication number: 20020102635Abstract: The invention relates to a thermophilic enzyme having &bgr;-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.Type: ApplicationFiled: August 6, 1999Publication date: August 1, 2002Inventors: IKUO MATSUI, KAZUHIKO ISHIKAWA, HIROYASU ISHIDA, YOSHITSUGU KOSUGI
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Publication number: 20020081697Abstract: The invention is directed to novel thermostable aminotransferases useful in synthesizing an amino acid derivative with high optical purity, and nucleic acids encoding the enzyme. The invention also includes antibodies that specifically bind to the aminotransferases of the invention.Type: ApplicationFiled: September 28, 2001Publication date: June 27, 2002Inventors: Ikuo Matsui, Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi, Eriko Matsui
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Patent number: 6391604Abstract: A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.Type: GrantFiled: March 3, 2000Date of Patent: May 21, 2002Assignee: Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi, Yasutaka Tahara
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Patent number: 6255081Abstract: The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.Type: GrantFiled: October 21, 1998Date of Patent: July 3, 2001Assignee: Director - General of Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Yoshitsugu Kosugi, Eriko Matsui, Satoko Kawasaki
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Patent number: 6251649Abstract: The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.Type: GrantFiled: September 3, 1998Date of Patent: June 26, 2001Assignee: Director - General of Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Yoshitsugu Kosugi, Eriko Matsui, Satoko Kawasaki
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Patent number: 6133012Abstract: An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.Type: GrantFiled: January 30, 1998Date of Patent: October 17, 2000Assignee: Director General of Agency of Industrial Science & TechnologyInventors: Kazuhiko Ishikawa, Ikuo Matsui, Hiroyasu Ishida, Yoshitsugu Kosugi, Katsuhiko Higuchi
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Patent number: 6001626Abstract: Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.Type: GrantFiled: December 1, 1997Date of Patent: December 14, 1999Assignee: Director-General of Agency of Industrial Science and TechnologyInventors: Yoshitsugu Kosugi, Katsuhiko Higuchi, Kazuhiko Ishikawa, Ikuo Matsui, Joh Yong-Goe
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Patent number: 5635378Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.Type: GrantFiled: June 6, 1995Date of Patent: June 3, 1997Assignee: Director-General of Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda
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Patent number: 5631149Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.Type: GrantFiled: June 6, 1995Date of Patent: May 20, 1997Assignee: Director-General Of Agency of Industrial Science and TechnologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda
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Patent number: 5538882Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.Type: GrantFiled: March 2, 1994Date of Patent: July 23, 1996Assignee: Director-General of Agency of Industrial Science and TechologyInventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda