Patents by Inventor Ilse Oberbaumer

Ilse Oberbaumer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Vectors and methods for the construction of cDNA libraries
    Patent number: 4985359
    Abstract: Vectors useful in the construction of cDNA libraries and methods for constructing these vectors are disclosed. According to the invention, a vector includes a polylinker region which is cut to linearize the vector. Oligo(dC) tails having terminal dideoxynucleotides are added to the two 3' ends of the vector. One oligo(dC) tail is removed and replaced with oligo(dT) to which poly(A) mRNA is annealed. The first complementary DNA strand is synthesized and tailed with oligo(dG), which anneals with the remaining oligo(dC) tail, thus circularizing the vector. The mRNA is digested and the second cDNA strand synthesized. Alternatively, the second cDNA strand can be synthesized before circularizing the vector. The resulting vector is used to transform microorganisms to create a cDNA library. The cDNA can be recovered by cleaving the vector at sites within the polylinker sequence.
    Type: Grant
    Filed: July 23, 1987
    Date of Patent: January 15, 1991
    Assignee: Ilse Oberbaumer
    Inventor: Ilse Oberbaumer