Abstract: Disclosed herein are systems and methods of estimating the autofluorescence (AF) signal and other non-target signals in each channel of a multi-channel image of a biological sample that is stained with one or more fluorescent labels. In some embodiments, the estimated autofluorescence signal can then be subtracted or masked from the multi-channel image. In some embodiments, the autofluorescence-removed multichannel image can then be use for further processing (e.g. image analysis, etc.).

Abstract: Disclosed herein are systems and methods of estimating the autofluorescence (AF) signal and other non-target signals in each channel of a multi-channel image of a biological sample that is stained with one or more fluorescent labels. In some embodiments, the estimated autofluorescence signal can then be subtracted or masked from the multi-channel image. In some embodiments, the autofluorescence-removed multichannel image can then be use for further processing (e.g. image analysis, etc.).

Abstract: System, method, and computer product for detection of objects. A plurality of image frames is extracted from an image data received from one or more imaging devices. At least one image frame is selected from the plurality of image frames. A determination is made whether the selected image frame contains at least one imaged object. Using at least one model, an intensity of pixels in the selected image frame is analyzed to determine presence of an anomaly associated with the at least one imaged object. Based on the analysis, a notification is generated upon determination that the anomaly is present in the selected image frame. The notification indicates that the at least one imaged object is suspicious.

Abstract: Systems including apparatus, methods, compositions, and kits for multiplexed analysis of biological systems using nonpositional and/or positional arrays of coded carriers.

Abstract: Systems for characterizing localization in biological cells.

Abstract: Systems, including apparatus and methods, for performing assays with adjustable fluid communication between samples.

Abstract: Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and/or microplates.

Abstract: Systems, including apparatus and methods, for performing assays with adjustable fluid communication between samples.

Abstract: Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and/or microplates.

Abstract: Systems using coded particles for multiplexed analysis of biological samples or reagents, in which the codes on the particles are at least partially defined by light-polarizing materials.

Abstract: The invention provides a multicolor LED light source for transmitted light illumination compatible with most microscopes. The light source provides spatially and angularly uniform monochromatic illumination in red, green, or blue color, or in their combinations. Switching of wavelengths is very fast, which is desirable for automated scanning of specimens. The light source is inexpensive and compact; it can be accommodated in the condenser space of a typical microscope, eliminating standard white light source, condenser and light filtering device. The invention also provides different means of shaping light for illumination of microscopic specimens. The invention also provides a method for acquiring full color images with the present light source and a monochrome camera.

Abstract: Systems using coded particles for multiplexed analysis of biological samples or reagents, in which the codes on the particles are at least partially defined by light-polarizing materials.

Abstract: Systems, including apparatus and methods, for performing assays with adjustable fluid communication between samples.

Abstract: Systems for determining and/or analyzing the distribution and dynamics of cellular components.

Abstract: A method for multiplexed detection and quantification of analytes by reacting them with probe molecules attached to specific and identifiable carriers. These carriers can be of different size, shape, color, and composition. Different probe molecules are attached to different types of carriers prior to analysis. After the reaction takes place, the carriers can be automatically analyzed. This invention obviates cumbersome instruments used for the deposition of probe molecules in geometrically defined arrays. In the present invention, the analytes are identified by their association with the defined carrier, and not (or not only) by their position. Moreover, the use of carriers provides a more homogenous and reproducible representation for probe molecules and reaction products than two-dimensional imprinted arrays or DNA chips.

Abstract: Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and/or microplates.

Abstract: Systems using coded particles for multiplexed analysis of biological samples or reagents, in which the codes on the particles are at least partially defined by light-polarizing materials.

Abstract: Systems, including methods, apparatus, compositions, and kits, for multiplexed analysis of different cell populations to measure a set of responses generated by the cell populations upon exposure to a condition in a shared fluid volume, to define a selective effect, if any, of the condition. The invention also provides databases that relate sets of responses measured from multiplexed analysis of cell populations in a plurality of shared fluid volumes to different conditions that generated each set of responses.

Abstract: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity.

Abstract: A method for multiplexed detection and quantification of analytes by reacting them with probe molecules attached to specific and identifiable carriers. These carriers can be of different size, shape, color, and composition. Different probe molecules are attached to different types of carriers prior to analysis. After the reaction takes place, the carriers can be automatically analyzed. This invention obviates cumbersome instruments used for the deposition of probe molecules in geometrically defined arrays. In the present invention, the analytes are identified by their association with the defined carrier, and not (or not only) by their position. Moreover, the use of carriers provides a more homogenous and reproducible representation for probe molecules and reaction products than two-dimensional imprinted arrays or DNA chips.