Abstract: The present disclosure relates generally to methods, compositions, and kits useful for detecting a multiplicity of bacterial genomes present in a sample (e.g., a biological sample obtained from a subject exhibiting one or more signs or symptoms of a bacterial infection). Specifically, the present disclosure relates to nucleic acid primers, primer sets, and multiplicities of primer sets that allow for broad (e.g., species non-specific) detection of bacterial genomes present in such a sample using loop-mediated isothermal amplification (LAMP).

Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.

Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.

Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.