Abstract: The present disclosure provides an effective chemical blocking agents for use with nylon membrane suitable for use in immunodiagnostic assays, flow-through assays and nucleic acid detection assays and methods of preparing and using same and to effective chemical blocking agents for treating unreinforced and reinforced nylon membrane and methods for the preparation thereof and the application thereof to unreinforced and reinforced nylon membrane, the chemical blocking agent including an effective amount of alkaline treated casein; an effective amount of polymer; and an effective amount of surfactant.

Abstract: The present application discloses a reinforced microporous membrane suitable for use in immunodiagnostic assays, the reinforced microporous membrane having lateral flow properties useful in immunodiagnostic assays applications, the reinforced nylon microporous membrane also including a scrim that provides specific lateral flow properties such that the combination produced thereby is useful in immunodiagnostic assays applications and to methods for using such reinforced microporous membrane in lateral flow immunodiagnostic assays applications and methods of preparing and using such membranes.