Patents by Inventor Inn Huam Yuk

Inn Huam Yuk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • PRODUCTION CELLS
    Publication number: 20240011066
    Abstract: Herein are reported modified mammalian cells wherein the expression of the MYC gene and one or more of the BAK, BAX, SIRT-1 and ICAM-1 genes has been reduced or eliminated as well as methods for the recombinant production of a heterologous polypeptide using a modified mammalian cell according to the current invention. Further reported are use of the reduction of the expression of the genes for increasing volumetric productivity, increasing cell volume, increasing viability and increasing the possible cultivation time without cell split.
    Type: Application
    Filed: April 19, 2023
    Publication date: January 11, 2024
    Applicants: Genentech, Inc., Hoffmann-La Roche Inc.
    Inventors: Shahram Misaghi, Amy Shen, Inn Huam Yuk, Gavin Christian Barnard, Simon Auslaender, Niels Bauer, Benedikt Oswald
  • CRISPR/Cas9 MULTIPLEX KNOCKOUT OF HOST CELL PROTEINS
    Publication number: 20230374497
    Abstract: The present disclosure relates to modified mammalian cells having reduced or eliminated expression of certain cellular proteins, CRISPR/Cas9 multiplex knockout strategies for making such cells, and methods of using such cells, e.g., in the context of cell-based therapy or as host cells in the production of a product of interest.
    Type: Application
    Filed: February 28, 2023
    Publication date: November 23, 2023
    Applicants: GENENTECH, INC., HOFFMANN-LA ROCHE INC.
    Inventors: Amy SHEN, Inn Huam Yuk, Peggy Wai No Ko, Shahram Misaghi, Simon Thomas Auslaender, Midori Greenwood-Goodwin, Michael Wilson Laird, Benedikt Alois Claudius Oswald
  • HIGH THROUGHPUT, FLUORESCENCE-BASED ESTERASE ACTIVITY ASSAY FOR ASSESSING POLYSORBATE DEGRADATION RISK DURING BIOPHARMACEUTICAL DEVELOPMENT
    Publication number: 20230348953
    Abstract: The present disclosure provides compositions, methods, and kits for detecting lipolytic activity. In some embodiments, the composition comprises an aqueous assay sample and an organic solvent, wherein the organic solvent comprises 4-methylumbellif-erryl caprylate (MU-C8). Also provided herein are methods for determining the stability of a protein preparation.
    Type: Application
    Filed: August 31, 2021
    Publication date: November 2, 2023
    Applicant: GENENTECH, INC.
    Inventors: Li YI, Adithi Chandrasekhara BHARGAVA, Inn Huam YUK
  • MODIFIED MAMMALIAN CELLS
    Publication number: 20210309989
    Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.
    Type: Application
    Filed: March 26, 2021
    Publication date: October 7, 2021
    Applicant: GENENTECH, INC.
    Inventors: Michael Wilson LAIRD, Shahram MISAGHI, Amy SHEN, Anthony TOMLINSON, Inn Huam YUK
  • CELL CULTURE STRATEGIES FOR MODULATING PROTEIN GLYCOSYLATION
    Publication number: 20210238644
    Abstract: The presently disclosed subject matter relates to cell culture media and cell culture strategies for modulating the glycosylation pattern, e.g., fucosylation and/or galactosylation pattern, of a glycoprotein of interest, e.g., an antibody, as well as cell culture and glycoprotein compositions prepared using such media and/or strategies.
    Type: Application
    Filed: February 10, 2021
    Publication date: August 5, 2021
    Applicant: GENENTECH, INC.
    Inventors: Inn Huam YUK, Patrick Daniel AHYOW, Anh Thuy NGUYEN DANG, Melissa Siu-Lan MUN, Kristen Leigh DOUGLAS, Robert David KISS
  • METHODS OF PROTEIN PRODUCTION
    Publication number: 20210230658
    Abstract: A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
    Type: Application
    Filed: April 2, 2021
    Publication date: July 29, 2021
    Applicant: GENENTECH, INC.
    Inventors: Inn Huam Yuk, Bradley Richard Snedecor, Dana Christian Andersen
  • Methods of protein production
    Patent number: 10982250
    Abstract: A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
    Type: Grant
    Filed: September 17, 2007
    Date of Patent: April 20, 2021
    Assignee: Genentech, Inc.
    Inventors: Inn Huam Yuk, Bradley Richard Snedecor, Dana Christian Andersen
  • METHODS OF PROTEIN PURIFICATION
    Publication number: 20100256336
    Abstract: A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
    Type: Application
    Filed: September 17, 2007
    Publication date: October 7, 2010
    Inventors: Inn Huam Yuk, Bradley Richard Snedecor, Dana Christian Andersen