Patents by Inventor Ioanna P. Petrounia

Ioanna P. Petrounia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Glucose 6-oxidases
    Patent number: 7220563
    Abstract: Glucose oxidase enzymes are provided, including novel variants of galactose oxidase enzymes. The polynucleotides that encode these novel variants can be expressed in recombinant host cell expression systems. The novel variant oxidase enzymes are capable of oxidizing compounds towards which wild-type galactose oxidase (e.g. D-galactose: oxygen 6-oxidoreductase, GAO; EC 1.1.3.9) has little or no activity. Preferred galactose oxidase variants are those which that have improved capability to oxidize secondary alcohols and/or D-glucose relative to the wild-type enzyme.
    Type: Grant
    Filed: February 27, 2003
    Date of Patent: May 22, 2007
    Assignee: California Institute of Technology
    Inventors: Frances H. Arnold, Lian-Hong Sun, Ioanna P. Petrounia
  • Directed evolution of galactose oxidase enzymes
    Patent number: 7115403
    Abstract: This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
    Type: Grant
    Filed: May 16, 2000
    Date of Patent: October 3, 2006
    Assignee: The California Institute of Technology
    Inventors: Frances H. Arnold, Ioanna P. Petrounia, Lianhong Sun
  • Directed evolution of oxidase enzymes
    Patent number: 7098010
    Abstract: This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantagoeusly produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
    Type: Grant
    Filed: November 27, 2000
    Date of Patent: August 29, 2006
    Assignee: California Institute of Technology
    Inventors: Frances H. Arnold, Ioanna P. Petrounia, Lianhong Sun
  • Novel glucose 6-oxidases
    Publication number: 20030228673
    Abstract: Glucose oxidase enzymes are provided, including novel variants of galactose oxidase enzymes. The polynucleotides that encode these novel variants can be expressed in recombinant host cell expression systems. The novel variant oxidase enzymes are capable of oxidizing compounds towards which wild-type galactose oxidase (e.g. D-galactose: oxygen 6-oxidoreductase, GAO; EC 1.1.3.9) has little or no activity. Preferred galactose oxidase variants are those which that have improved capability to oxidize secondary alcohols and/or D-glucose relative to the wild-type enzyme.
    Type: Application
    Filed: February 27, 2003
    Publication date: December 11, 2003
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Frances H. Arnold, Lian-Hong Sun, Ioanna P. Petrounia