Patents by Inventor Ioannis PAPAPETRIDIS
Ioannis PAPAPETRIDIS has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11667886Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.Type: GrantFiled: June 4, 2018Date of Patent: June 6, 2023Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Jacobus Thomas Pronk
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Patent number: 11198847Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.Type: GrantFiled: January 31, 2019Date of Patent: December 14, 2021Assignee: DSM IP ASSETS B.V.Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Maarten Dirk Verhoeven, Ioannis Papapetridis
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Patent number: 11186850Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: GrantFiled: April 24, 2020Date of Patent: November 30, 2021Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Patent number: 11136600Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.Type: GrantFiled: October 3, 2016Date of Patent: October 5, 2021Assignee: DSM IP Assets B.V.Inventors: Ioannis Papapetridis, Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Paul Klaassen
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Patent number: 10913928Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.Type: GrantFiled: December 18, 2017Date of Patent: February 9, 2021Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Publication number: 20200377846Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d?) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genType: ApplicationFiled: January 31, 2019Publication date: December 3, 2020Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Maarten Dirk VERHOEVEN, Ioannis PAPAPETRIDIS
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Publication number: 20200255871Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: ApplicationFiled: April 24, 2020Publication date: August 13, 2020Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Paulus Petrus DE WAAL, Niels LAURET, Maarten Dirk VERHOEVEN, Jozef Petrus Johannes SCHMITZ
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Patent number: 10689670Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: GrantFiled: June 13, 2017Date of Patent: June 23, 2020Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Publication number: 20200157489Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.Type: ApplicationFiled: June 4, 2018Publication date: May 21, 2020Inventors: Ioannis PAPAPETRIDIS, Jacobus Thomas PRONK
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Publication number: 20200095536Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.Type: ApplicationFiled: December 18, 2017Publication date: March 26, 2020Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK
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Publication number: 20190249201Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: ApplicationFiled: June 13, 2017Publication date: August 15, 2019Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Paulus Petrus DE WAAL, Niels LAURET, Maarten Dirk VERHOEVEN, Jozef Petrus Johannes SCHMITZ
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Publication number: 20180291404Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.Type: ApplicationFiled: October 3, 2016Publication date: October 11, 2018Applicant: DSM IP Assets B.V.Inventors: Ioannis PAPAPETRIDIS, Jacobus THomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Paul KLAASSEN