Abstract: This disclosure describes recombinant angiotensin-converting enzyme II (ACE2) polypeptides, fusion proteins, and compositions thereof having improved binding affinity for the SARS-CoV-2 spike protein receptor binding domain relative to wild-type ACE2. Also provided are methods of using the recombinant ACE2 polypeptides, fusion proteins, and compositions thereof for treating subjects infected with a SARS-CoV-2 virus (i.e., subjects with COVID-19), subjects having symptoms suggestive of a SARS-CoV-2 infection, and subjects exposed to or at risk of exposure to SARS-CoV-2 virus. Other virus infections may also be treated.

Abstract: Provided herein are protein biosensors, fusion proteins, compositions, and methods that are useful in detecting SARS-CoV-2 viruses in a sample from a subject. The viral detection assays described herein are solution-based, rapid, and quantitative. The protein biosensors and fusion proteins herein are able to bind to SARS-CoV-2 viral proteins. Use of the fusion proteins in proximity assays (e.g., split reporter assays) allows sensitive detection of SARS-CoV-2 virus in samples.

Abstract: A cultureware system is directed to maintaining viability of mammalian cells, and includes a disposable holding container having a cell receiver with a cell-receiving surface. The cell receiver consists of a cell-adhesion inducement material including a polystyrene material and a glass material. The system further includes a polytetrafluoroethylene (PTFE) coating lining the cell-adhesion inducement material of the cell-receiving surface, and a culture of adherent mammalian cells located within the cell receiver on the PTFE coating. In response to attachment interaction between the mammalian cells and the PTFE coating, a decreased cell adhesion results in a cell viability rate of at least about 60% to about 70% over a 72-hour culturing period, the cell viability rate being under 90% over the 72-hour culturing period if, in the absence of the PTFE coating, the mammalian cells are located directly on the cell-adhesion inducement material.

Abstract: This invention relates to modified bacteriophage useful for the delivery of macromolecular biopolymers and nanoparticles to target cells, e.g., disease cells, and their use in cell-selective identification and imaging, as well as the treatment and prevention of diseases and other medical conditions.