Patents by Inventor Irina Bruck
Irina Bruck has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9977026Abstract: Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells.Type: GrantFiled: July 27, 2016Date of Patent: May 22, 2018Assignee: The Florida State University Research Foundation, Inc.Inventors: Daniel L. Kaplan, Irina Bruck
-
Publication number: 20170030914Abstract: Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells.Type: ApplicationFiled: July 27, 2016Publication date: February 2, 2017Inventors: Daniel L. Kaplan, Irina Bruck
-
Publication number: 20090240035Abstract: The present invention relates to alpha-large, alpha-small, delta, delta prime, tau, beta, SSB, DnaG, and DnaB encoding genes from Gram positive bacterium, preferably Streptococcus and Staphylococcus bacterium. The formation of functional polymerase as well as the use of such a polymerase in sequencing and amplification is also disclosed. The individual genes and proteins or polypeptides are useful in identification of compounds with antibiotic activity.Type: ApplicationFiled: June 25, 2008Publication date: September 24, 2009Inventors: Michael E. O'Donnell, Irina Bruck, Dan Zhang, Richard Whipple
-
Patent number: 7432365Abstract: The present invention relates to beta protein-encoding genes from Gram positive bacterium. Expression systems and host cells containing these genes are also disclosed.Type: GrantFiled: July 28, 2000Date of Patent: October 7, 2008Inventors: Michael E. O'Donnell, Irina Bruck, Dan Zhang, Richard Whipple
-
Publication number: 20050153299Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: July 14, 2005Inventors: Michael O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20050112580Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: May 26, 2005Inventors: Michael O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20050100920Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: May 12, 2005Inventors: Michael O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20050048510Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: March 3, 2005Inventors: Michael O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040197796Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: October 7, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040110210Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: June 10, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040106137Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polyrnerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: June 3, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040081995Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: April 29, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040077012Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: April 22, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040048309Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: March 11, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040043414Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: March 4, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040043415Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: March 4, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040038289Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: February 26, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
-
Publication number: 20040038290Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 25, 2003Publication date: February 26, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan