Abstract: Provided herein is the first viable galactosyltransferase (Gal) knock-out sheep having a deletion or mutation of alpha-1,3-galactosyltransferase (GGTA1) gene and methods of making the same. Also provided are methods of screening a biological implant for stimulation of an antibody-mediated inflammatory response to a Gal antigen by implanting the biological implant into a recipient Gal knock-out animal and detecting signs of antibody-mediated inflammatory response in the recipient Gal knock-out animal. Further provided is a method of implanting a biological implant into a human subject by screening a first biological implant for signs of antibody-mediated inflammatory response in a recipient Gal knock-out animal and, upon detecting minimal or no signs of antibody mediated inflammatory response in the recipient Gal knock-out animal, implanting a second biological implant into the human subject, wherein the second biological implant is comparable to the first biological implant.

Abstract: Herein provided are a model and method for a transgenic a bovidae having an TGF-?1 gene inserted into the bovidae genome and capable of expressing higher than normal levels of TGF-?1 in cardiac muscle.

Abstract: Herein provided are a model and method for a transgenic a bovidae having an TGF-?1 gene inserted into the bovidae genome and capable of expressing higher than normal levels of TGF-?1 in cardiac muscle.

Abstract: Methods for the efficient production of cloned porcine fetuses/piglets following the production of cloned embryos, including culture of the embryos for extended periods prior to transfer of the embryos into the uterus of the recipient. Transfer can be accomplished surgically or through less-invasive laparoscopic or non-surgical transfer.