Abstract: The present invention relates to a reaction unit (10) configured to receive a reaction solution or culture media and configured to be placed inside a thermocycler, said reaction unit (10) comprising an elongated hollow body (12) extending along a flowing axis X, the hollow body (12) thus displaying a first opening (14) at its first extremity (16) and a second opening (18) at its second extremity (20). The walls of the hollow body (12) are at least partially made of a thermally conductive material. The reaction unit (10) further comprises at least one filter element (22) extending inside the hollow body (12), the filter element (22) being sealingly secured to the walls of the hollow body (12) over its complete circumference, leading any fluid flowing from the first opening (14) to the second opening (18) to cross the at least one filter element (22).

Abstract: Thermocycling multistep reaction device comprising a reaction plate (12) comprising at least one well (14) and at least one internal fluid circulation channel arranged around the at least one well (14), an evacuation system (16), a first circuit being aimed, when activated, at driving a first heat transfer fluid through the at least one internal fluid circulation channel, a second circuit being aimed, when activated, at driving a second heat transfer fluid through the at least one internal fluid circulation channel, and a control unit. The temperature inside the at least one well (14) is regulated and controlled by means of the control unit which selectively activates the circuits, each well (14) is connected to the evacuation system (16) by means of at least one filter membrane.

Abstract: Thermocycling multistep reactions device (10) comprising: a reaction plate (12) comprising at least one well (14), at least one internal thermoregulating fluid circulation circuit configured to be arranged in direct contact with the at least one well (14), an evacuation system, a control unit configured to manage the temperature of the internal thermoregulating fluid circuit. The temperature inside the at least one well (14) is regulated and controlled by means of the control unit. Each well (14) is connected to the evacuation system by means of at least one filter membrane, and the thermoregulating fluid circuit is made of a heat conducting material while the reaction plate is made (12) of a polymerous material.

Abstract: The invention relates to methods for template-independent synthesis of nucleic acids, comprising iteratively contacting an initiator sequence comprising a 3?-end nucleotide with a free 3?-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3-hydroxyl group of the 3-end nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of template-independent terminal nucleotidyl transferase activity but are devoid of a template-independent primase activity.

Abstract: The invention relates to the field of nucleic acid synthesis or sequencing, more specifically to methods for ab-initio synthesis of nucleic acids, comprising contacting a nucleotide with a free 3?-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3?-hydroxyl group of the nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of both ab-initio single-stranded nucleic acid synthesis activity and template-independent terminal nucleotidyl transferase activity.

Abstract: The invention relates to mutant DNA polymerases of the Pol theta subfamily capable of performing non-templated nucleic acid extension, or of a functional fragment of such a polymerase, methods of producing these mutant DNA polymerases, kits and methods of using these mutant DNA polymerases.

Abstract: The invention relates to mutant DNA polymerases of the Pol theta subfamily capable of performing non-templated nucleic acid extension, or of a functional fragment of such a polymerase, methods of producing these mutant DNA polymerases, kits and methods of using these mutant DNA polymerases.

Abstract: The invention relates to mutant DNA polymerases of the Pol theta subfamily capable of performing non-templated nucleic acid extension, or of a functional fragment of such a polymerase, methods of producing these mutant DNA polymerases, kits and methods of using these mutant DNA polymerases.