Patents by Inventor Irudaya Charles

Irudaya Charles has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAI, and stabilized forms thereof
    Patent number: 10900038
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: January 26, 2021
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Maylin Magdaleno, Alexander Vlassov, Christopher Burnett
  • SIRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAI, AND STABILIZED FORMS THEREOF
    Publication number: 20190376062
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: May 10, 2019
    Publication date: December 12, 2019
    Inventors: Nitin Puri, Irudaya Charles, Susan Maylin Magdaleno, Alexander Vlassov, Christopher Burnett
  • siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
    Patent number: 10329564
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: September 5, 2017
    Date of Patent: June 25, 2019
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Maylin Magdaleno, Alexander Vlassov, Christopher Burnett
  • SIRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAI, AND STABILIZED FORMS THEREOF
    Publication number: 20180057815
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: September 5, 2017
    Publication date: March 1, 2018
    Inventors: Nitin PURI, Irudaya CHARLES, Susan Maylin MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAI, and stabilized forms thereof
    Patent number: 9771583
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: September 26, 2017
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Magdaleno, Alexander Vlassov, Christopher Burnett
  • SIRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAI, AND STABILIZED FORMS THEREOF
    Publication number: 20160230170
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: February 4, 2016
    Publication date: August 11, 2016
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • RNAi sequence-independent modification formats, and stabilized forms thereof
    Patent number: 9284551
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: March 15, 2016
    Assignee: Applied Biosystems, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Magdaleno, Alexander Vlassov, Chris Burnett
  • SiRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
    Patent number: 9273312
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: March 1, 2016
    Assignee: Applied Biosystems, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Magdaleno, Alexander Vlassov, Christopher Burnett
  • SiRNA Sequence-Independent Modification Formats for Reducing Off-Target Phenotypic Effects in RNAi, and Stabilized Forms Thereof
    Publication number: 20140094501
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: August 23, 2013
    Publication date: April 3, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • RNAi Sequence-Independent Modification Formats, and Stabilized Forms Thereof
    Publication number: 20130244327
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: March 1, 2013
    Publication date: September 19, 2013
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Chris BURNETT
  • siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
    Patent number: 8524681
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: March 19, 2010
    Date of Patent: September 3, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Magdaleno, Alexander Vlassov, Chris Burnett
  • Preparation and isolation of 5? capped MRNA
    Patent number: 8344118
    Abstract: The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: January 1, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Anilkumar Kore, Shanmugasundaram Muthian, Irudaya Charles
  • PREPARATION AND ISOLATION OF 5' CAPPED MRNA
    Publication number: 20120156751
    Abstract: The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.
    Type: Application
    Filed: December 13, 2011
    Publication date: June 21, 2012
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Anilkumar Kore, Shanmugasundaram Muthian, Irudaya Charles
  • Preparation and isolation of 5? capped mRNA
    Patent number: 8093367
    Abstract: The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: January 10, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: Anilkumar Kore, Shanmugasundaram Muthian, Irudaya Charles
  • Preparation And Isolation of 5' Capped mRNA
    Publication number: 20100304389
    Abstract: The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.
    Type: Application
    Filed: April 30, 2010
    Publication date: December 2, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Anilkumar KORE, Shanmugasundaram MUTHIAN, Irudaya CHARLES
  • SiRNA Sequence-Independent Modification Formats for Reducing Off-Target Phenotypic Effects in RNAi, and Stabilized Forms Thereof
    Publication number: 20100222414
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: March 19, 2010
    Publication date: September 2, 2010
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Chris BURNETT