Abstract: Improved methods of producing nucleic acid molecules, proteins and peptides in host cells and genetically engineered plants, vectors and constructs therefor.

Abstract: Methods, reagents and compositions for providing more accurate and reliable genetic modification are provided. In particular, a nucleic acid encoding a fusion protein comprising a 5? to 3? DNA exonuclease domain and an RNA binding domain is described. Also provided are methods, reagents and compositions for in vivo genetic modification of the genome of a human or animal cell. Furthermore, the present application relates to uses of the said methods, reagents and compositions in the treatment of disease and production of transgenic animals.

Abstract: Methods, reagents and compositions for providing more accurate and reliable genetic modification are provided. In particular a nucleic acid encoding a fusion protein comprising an endonuclease domain and a binding domain for an origin of replication is described. Also provided are methods, reagents and compositions for in vivo genetic modification of the genome of a non-animal cell or organism. Furthermore, the present application relates to uses of the said methods, reagents and compositions for introducing desirable traits to non-animal organisms or ameliorating or removing non-desirable traits in these organisms including in the treatment of disease.

Abstract: An isolated polynucleotide sequence that encodes for a multi domain recombination protein, comprising a donor DNA-Binding domain (BDB); and a chromosomal binding domain (CBD). A plant cell can be transformed by introducing into the cell a first nucleic acid sequence, which comprises a plant nuclear promoter operably linked to a first nucleic acid sequence comprising the stated polynucleotide sequence, and a second nucleic acid sequence, which comprises a donor nucleic acid sequence and at least two flanking sequences capable of binding to a donor binding domain of the protein, the sequences being located adjacent to each end of the donor polynucleotide sequence forming left and right borders thereto.

Abstract: An isolated polynucleotide sequence that encodes for a multi domain recombination protein, comprising a donor DNA-Binding domain (BDB); and a chromosomal binding domain (CBD). A plant cell can be transformed by introducing into the cell a first nucleic acid sequence, which comprises a plant nuclear promoter operably linked to a first nucleic acid sequence comprising the stated polynucleotide sequence, and a second nucleic acid sequence, which comprises a donor nucleic acid sequence and at least two flanking sequences capable of binding to a donor binding domain of the protein, the sequences being located adjacent to each end of the donor polynucleotide sequence forming left and right borders thereto.

Abstract: Improved methods of producing nucleic acid molecules, proteins and peptides in host cells and genetically engineered plants, vectors and constructs therefor.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: Method for heterologous RNA species and protein production in plant cell mitochondria comprising introducing into plant cells nucleic acid components that encode heterologous proteins/RNAS under the control of promoters operative in mitochondria, vectors, host cells, plants and uses thereof.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: An isolated polynucleotide sequence that encodes for a multi domain recombination protein, comprising a donor DNA-Binding domain (BDB); and a chromosomal binding domain (CBD). A plant cell can be transformed by introducing into the cell a first nucleic acid sequence, which comprises a plant nuclear promoter operably linked to a first nucleic acid sequence comprising the stated polynucleotide sequence, and a second nucleic acid sequence, which comprises a donor nucleic acid sequence and at least two flanking sequences capable of binding to a donor binding domain of the protein, the sequences being located adjacent to each end of the donor polynucleotide sequence forming left and right borders thereto.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: Method for heterologous RNA species and protein production in plant cell mitochondria comprising introducing into plant cells nucleic acid components that encode heterologous proteins/RNAS under the control of promoters operative in mitochondria, vectors, host cells, plants and uses thereof.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.