Abstract: Provided is a bending device that includes a processing part having two protruding parts that protrude from an end surface of a pillar part and that bend a wire, a rotary part that supports the processing parts and rotates about an axis intersecting the length direction of the pillar part, an accommodating part that is cylindrical and accommodates the rotary part at an end part of the accommodating part so as to be rotatable, and a mechanism that rotates the accommodating part about an axis of the accommodating part.

Abstract: An example machine comprising a processing part having two protruding parts that protrude from an end surface of a pillar part and that bend a wire, wherein at least one of the protruding parts has a curved surface part, and the wire is disposed between the two protruding parts, the pillar part is rotated around an axis of the pillar part to bend the wire along the curved surface part, the bending device comprises plural processing parts, curvature of the curved surface part of the protruding part in each processing part are different, and the bending device further comprises: a rotary part that supports the processing parts and rotates about an axis intersecting the length direction of the pillar part; an accommodating part that is cylindrical and accommodates the rotary part at an end part of the accommodating part so as to be rotatable; and a mechanism that rotates the accommodating part about an axis of the accommodating part.

Abstract: Under the regulation of a methanol-inducible alcohol oxidase gene (AOX1) promoter and a transcription termination sequence originating from Pichia pastoris, Pichia yeast, which has been transformed with an expression vector containing an MK family protein gene ligated to an a1 factor signal sequence originating from Saccharomyces cerevisiae, is cultured and an intact MK family protein is thus expressed and secreted in a large amount into the culture supernatant.