Abstract: The present invention is directed to directly measure distribution in vivo and the frequency of generation in vivo of DNA strand breaks which induce cell death and mutations. The present inventors accomplished the present invention by providing a method for detecting a DNA strand break in a sample, which comprises a step of binding a PprA protein derived from Deinococcus radiodurans to a DNA strand break and a step of detecting the PprA protein which is bound to the DNA strand break; as well as by providing a kit for detecting a DNA strand break in a sample which comprises PprA proteins derived from Deinococcus radiodurans and a means for detecting a PprA protein which is bound to a DNA strand break.

Abstract: The present invention is directed to directly measure distribution in vivo and the frequency of generation in vivo of DNA strand breaks which induce cell death and mutations. The present inventors accomplished the present invention by providing a method for detecting a DNA strand break in a sample, which comprises a step of binding a PprA protein derived from Deinococcus radiodurans to a DNA strand break and a step of detecting the PprA protein which is bound to the DNA strand break; as well as by providing a kit for detecting a DNA strand break in a sample which comprises PprA proteins derived from Deinococcus radiodurans and a means for detecting a PprA protein which is bound to a DNA strand break.

Abstract: The endogenous plasmid pUE30 from radioresistant bacterium D. radiopugnans strain ATCC19172 or a derivative thereof is used as a vector that can autonomously replicate in bacteria of the genus Deinococcus. It may be used to construct a shuttle vector that also contains a plasmid capable of autonomous replication in E. coli or a derivative thereof and which can replicate in both a bacterium of the genus Deinococcus and E. coli.

Abstract: The endogenous plasmid pUE30 from radioresistant bacterium D. radiopugnans strain ATCC19172 or a derivative thereof is used as a vector that can autonomously replicate in bacteria of the genus Deinococcus. It may be used to construct a shuttle vector that also contains a plasmid capable of autonomous replication in E. coli or a derivative thereof and which can replicate in both a bacterium of the genus Deinococcus and E. coli.

Abstract: Provided are a protein having DNA repair reaction promoting activity which consists of the amino acid sequence represented by SEQ ID NO: 1 or a modified product of that amino acid sequence, a nucleotide sequence coding for said protein having DNA repair promoting activity, a recombinant DNA having said nucleotide sequence inserted into vector DNA, a transformant containing said recombinant DNA, a process for producing a protein having DNA repair promoting activity comprising the steps of cultivating said transformant in a medium and harvesting a protein having DNA repair promoting activity from the culture, and an antibody binding to said protein.