Patents by Inventor Itsuo Yokoyama

Itsuo Yokoyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA coding for endo-?-galactosidase
    Patent number: 7393672
    Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.
    Type: Grant
    Filed: April 20, 2006
    Date of Patent: July 1, 2008
    Assignee: Seikagaku Corporation
    Inventors: Takashi Muramatsu, Haruko Ogawa, Hisako Muramatsu, Takaaki Kobayashi, Itsuo Yokoyama
  • DNA coding for endo-beta-galactosidase
    Publication number: 20060177427
    Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.
    Type: Application
    Filed: April 20, 2006
    Publication date: August 10, 2006
    Inventors: Takashi Muramatsu, Haruko Ogawa, Hisako Muramatsu, Takaaki Kobayashi, Itsuo Yokoyama
  • DNA encoding endo-?-galactosidase
    Patent number: 7060483
    Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.
    Type: Grant
    Filed: October 10, 2000
    Date of Patent: June 13, 2006
    Assignee: Seikagaku Corporation
    Inventors: Takashi Muramatsu, Haruko Ogawa, Hisako Muramatsu, Takaaki Kobayashi, Itsuo Yokoyama