Abstract: The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

Abstract: The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

Abstract: An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.

Abstract: The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

Abstract: A device for transferring small drops of liquid from one or more liquid holders to one or more liquid destinations is disclosed. The device includes a support member and a plurality of fluid transfer members extending from the support member. Each fluid transfer member includes a flexible liquid transfer end.

Abstract: An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.

Abstract: A method of forming a chromatography cartridge includes providing a flexible-walled tube, placing a first end cap within the tube in sealing engagement with the tube wall, forming a media bed within the tube supported by the first end cap, cooling a second end cap to thermally contract the second end cap, placing the contracted second end cap within the tube and against the media bed, and sealing the second end cap to the tube wall by thermal expansion of the second end cap upon warming. The expansion of the second end cap causes the tube wall to deform. A chromatography column includes a flexible-walled tube containing chromatography media, and an end cap positioned within the tube. The end cap has a dimension greater than an inner dimension of the tube such that the tube is locally deformed by the end cap.

Abstract: A chromatography column includes a tubular member with an inlet end and a slidable porous member that bounds a chromatography media. The porous member is spaced sufficiently from the inlet end to define a receiving region.

Abstract: A chromatography sample module including a flow-through member having an inlet and an outlet, chromatography media within the flow-through member, and a sample carried on the media. The module can fit within a chromatography column, and a plurality of modules can be arranged in an array in a rack to facilitate processing of multiple samples.

Abstract: A chromatography sample module including a flow-through member having an inlet and an outlet, chromatography media within the flow-through member, and a sample carried on the media. The module can fit within a chromatography column, and a plurality of modules can be arranged in an array in a rack to facilitate processing of multiple samples.

Abstract: A method of forming a chromatography cartridge includes providing a flexible-walled tube, placing a first end cap within the tube in sealing engagement with the tube wall, forming a media bed within the tube supported by the first end cap, cooling a second end cap to thermally contract the second end cap, placing the contracted second end cap within the tube and against the media bed, and sealing the second end cap to the tube wall by thermal expansion of the second end cap upon warming. The expansion of the second end cap causes the tube wall to deform. A chromatography column includes a flexible-walled tube containing chromatography media, and an end cap positioned within the tube. The end cap has a dimension greater than an inner dimension of the tube such that the tube is locally deformed by the end cap.