Abstract: A spiral spring for a horological resonator mechanism, including a flexible strip (2) coiled on itself into several turns The spring (1) including an element for adjusting its stiffness, which includes a first elongate flexible element (5), and a second elongate flexible element (15) arranged in series with the strip (2), each elongate flexible element (5) connecting the same end (4) of the strip (2) to a fixed support (11), so as to add an additional stiffness to the strip (2), each elongate flexible element (5) preferably having a stiffness higher than that of the strip (2). A prestressing element (6) applies at least two different adjustable efforts, the two efforts being applied on the first elongate flexible element (5) so as to make the stiffness of the first elongate flexible element (5) vary according to the prestress level.

Abstract: A spiral spring for a horological resonator mechanism, including a flexible strip (2) coiled on itself into several turns, the strip (2) having a predefined stiffness. The spring (1) include an element for adjusting its stiffness, including a unique elongate flexible element (5) arranged in series with the strip (2), and connecting one end (4) of said strip (2) to a fixed support (11), to add additional stiffness. The flexible element (5) has a stiffness higher than that of the strip (2). A prestressing element (6) applies two different efforts on the element (5), and includes a first lever (8) attached to the end (4) of the strip (2) to allow adjusting a first effort, and a second lever (8) also attached to the end (4) of the strip (2) to adjust a second effort independently of the first effort.

Abstract: The invention relates to an elastic retaining member (1) for fixing a timepiece component (2) on a support element (3), comprising an opening (5) into which said support element (3) can be inserted, the retaining member (1) comprising rigid arms (6) and elastic arms (7) defined between connection areas (9) contributing to ensure elastic clamping of the support element (3) in the opening (5) each rigid arm (6) being provided with two flat contact areas (8) of the retaining member (1) able to cooperate with corresponding convex contact portions (10) of the support element (3).

Abstract: An elastic retaining member for fixing a timepiece component on a support element includes an opening into which the support element can be inserted, and rigid arms and elastic arms defined between connection areas of the member. The rigid arms and the elastic arms are configured to ensure elastic clamping of the support element in the opening, each of the rigid arms being provided with a single flat contact area and configured to cooperate with a corresponding convex contact portion of the support element.

Abstract: Techniques are described for exporting observability data related to execution of a service from a first cloud environment to a second cloud environment. The service is executed in the first cloud environment for a customer of a second cloud environment. Observability data associated with execution of the service is collected in the first cloud environment for the customer of the second cloud environment. The observability data comprises one or more metrics associated with the execution of the service. The observability data collected from the first cloud environment is communicated to the second cloud environment to enable a user associated with the customer of the second cloud environment to access the observability data via the second cloud environment.

Abstract: A multi-cloud infrastructure included in a first cloud environment provided by a first cloud services provider generates a set of one or more graphical user interfaces for each of a plurality of external cloud environments that are provided by a plurality of external cloud services providers. The set of one or more graphical user interfaces for an external cloud environment of the plurality of external cloud environments is generated based upon a native graphical user interface provided by the external cloud environment. Responsive to a request received by the first cloud environment and from a first external cloud environment of the plurality of external cloud environments, providing as a response to the request, a first graphical user interface from the set of one or more graphical user interfaces generated for the first external cloud environment.

Abstract: The present disclosure in some aspects relates to methods and compositions for detecting and quantifying multiple analytes present in a biological sample by rolling circle amplification (RCA). In some aspects, the methods and compositions provided herein address issues associated with control of signal spot size and intensity for RCA products (RCPs) in a sample. In some aspects, provided herein are methods and compositions (e.g., probes and/or reaction mixtures) for slowing down RCA and/or decreasing the size of RCA products. In some embodiments, nucleotides or nucleotide analogs having a hydrophobic group (e.g., a hydrophobic modification on the base) are included in nucleotides for RCA and incorporated into the RCP product, leading to reduction of RCP size without the need of crosslinking an incorporated hydrophobic nucleotide or nucleotide analog to the RCP itself or another molecule.

Abstract: A balance-spring, particularly for a timepiece resonator mechanism. An adjustment device include a single elongated flexible element (5) arranged in series of strip (2), connecting one end (4, 9) of said strip (2) to a fixed support (11), to add an additional stiffness to the strip (2), and having a stiffness greater than that of the strip (2). A prestressing means (6) applies at least two different stresses on the elongated flexible element (5), the first stress being provided by a tensile/compressive force directed substantially in the longitudinal direction FL of the elongated flexible element (5), and the second stress being provided, either by a force directed substantially in a direction substantially orthogonal FT to the longitudinal direction of the elongated flexible element (5), or by a torque M, preferably a bending moment, in such a way as to vary the stiffness of the elongated flexible element (5).

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

Abstract: The present disclosure in some aspects relate to a direct RNA (dRNA) detection approach incorporating the use of circularizable probes (e.g., padlock probes) having asymmetric arms, rolling circle amplification of the ligated circularizable probes, and in situ detection (e.g., using hybridization-based in situ sequencing) for multiplexed spatial analysis of nucleic acid sequences (e.g., short sequences such as SNPs and point mutations) in a biological sample. In some aspects, compositions and methods disclosed herein improve detection specificity, reduce false positive signal detection, and/or maintain or improve detection efficiency.

Abstract: A balance for a horological movement, including rigid parts including a hub defining the pivot axis of the balance, a felloe sector, at least one arm connecting the felloe sector to the hub, and including a slot for receiving and gripping an inertia-block in position, the slot opening into a housing delimited on the one hand by a rigid part of the balance, and on the other hand an elastic arm including a first end integral with a rigid part of the balance, and a second free distal end. The elastic arm has a hook-shaped body, the free distal end of the hook being parallel to a part of the balance having a rigidity greater than or equal to that of the elastic arm.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures, such as RCPs, in the biological sample without the use of exogenously added oligonucleotide compaction probes. In some embodiments, provided herein are methods involving the use of self-hybridizing hybridizing regions for compacting and/or stabilizing nucleic acid concatemers (e.g., RCPs). In some embodiments, dynamic inter-strand annealing between tandem units of an RCP is used for compaction and/or stabilization. In some embodiments, short palindromic regions in an RCP are used for compaction and/or stabilization.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address issues associated with the heterogeneity of analyte abundance (e.g., gene expression levels) and variations among reactions at different locations of a sample (e.g., amplification reaction starting earlier at one location than another location). In some aspects, a method disclosed herein provides a tighter distribution of signal spot size and intensity in a sample, as compared to methods that result in a wide and heterogeneous size and intensity distribution of signal spots.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein allow for detection of a target sequence by rolling circle amplification without requiring a ligation step and without sacrificing specificity (e.g., rolling circle amplification occurs only for circular probes and/or hairpin molecules specifically hybridized to a target sequence).

Abstract: The present disclosure relates in some aspects to methods and compositions for analysis of a target nucleic acid, such as in situ detection of a region of interest in a polynucleotide in a tissue sample. In some embodiments, provided herein are templated ligation probes (e.g., RNA-templated ligation probes) and selector probes for generation of a circularized ligated probe comprising an insertion sequence of a selector probe, wherein the circularized ligated probe is amplified in a rolling circle amplification reaction to generate a product that is detected in the sample.

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, provided herein are methods and compositions for detecting a region of interest in a target nucleic acid, wherein hybridization between an interrogatory region of a probe and a region of interest of the target nucleic acid is blocked by a blocking strand unless the interrogatory region is complementary to the region of interest. In some aspects, the methods provided herein increase specificity of detecting a region of interest in a target nucleic acid (e.g., a SNP in an RNA molecule). In some aspects, the presence, amount, and/or identity of a region of interest in a target nucleic acid is analyzed in situ. Also provided are polynucleotides, sets of polynucleotides, compositions, and kits for use in accordance with the methods, for example for RNA-targeting padlock probe-mediated SNP detection.

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, provided herein are methods and compositions for detecting a region of interest in a target nucleic acid.

Abstract: A balance for a horological movement, including rigid parts constituted by a hub defining the pivot axis of the balance, at least one felloe sector, at least one arm connecting the at least one felloe sector to said hub, and including a slot for receiving and gripping in position an inertia-block, the slot opening into a housing delimited on the one hand by a rigid part of the balance, and on the other hand an elastic arm including a first end integral with a rigid part of the balance, and a second free distal end. The elastic arm can have a body of a non-constant section, a part of the body having a greater thickness than the rest of the elastic arm so as to have a larger volume of material under stress and store a maximum of elastic energy.

Abstract: A sample analysis device (9) comprises a sample receiving well (7) configured to receive a liquid sample (4) comprising rolling circle amplification products (RCPs) (5). The device (9) also comprises a filter membrane (1) permeable for liquid of the liquid sample (4) but substantially impermeable for RCPs (5) in the liquid sample (4). The sample receiving well (7) is defined in the filter membrane (1) or is in connection with the filter membrane (1). An absorption layer (3) is in liquid connection with the filter membrane (1) and is configured to suck liquid from the liquid sample (4) received in the sample receiving well (7) through the filter membrane (1) by capillary force. The dimensions of the sample receiving well (7) are restricted to a field of view of an optical sensing device (10).

Abstract: A holding member for fixing a timepiece component on support elements of different cross section includes an opening into which each support element can be inserted, the holding member having structural elements together forming a body configured to ensure mounting of each support element in the opening. Each of the structural elements includes a first structural sub-element and a second structural sub-element, the first structural sub-element including a volume of material greater than a volume of material constituting the second structural sub-element. The holding member includes a connecting portion ensuring the mounting of each of the support elements in the holding member, the portion being defined on an inner face of the first structural sub-element.